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首页> 外文期刊>Journal of Cellular Physiology >Specific binding of leukemia inhibitory factor to murine myoblasts in culture.
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Specific binding of leukemia inhibitory factor to murine myoblasts in culture.

机译：特定的绑定的白血病抑制因子小鼠成肌细胞在文化。

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Leukemia inhibitory factor (LIF) is a member of the cytokine family of growth factors. It has been shown to exert a variety of actions on a diverse range of cell types, including neuronal, bone, and hemopoietic cells (Hilton, 1992, Trends Biochem. Sci., 17:72-76). In many of these cell types, studies have indicated the presence of specific receptors for LIF (Godard et al., 1982, J. Biol. Chem., 267: 3214-3222; Hilton et al., Proc. Natl. Acad. Sci. USA, 85:5971-5975; Hilton and Nicola, 1992, J. Biol. Chem., 267:10238-10247.). The mechanism by which these receptors act is believed to involve tyrosine phosphorylation and the signal transducing receptor component gp130. We have previously shown that LIF is capable of inducing both human and murine myoblasts to proliferate in culture (Austin et al., 1992, J. Neurol. Sci., 112:185-191). We now report that LIF binds specifically to receptors on the surface of myoblasts, with an equilibrium dissociation constant of 400 pM and the number of receptors per cell varies with cell density. Binding competition studies showed that LIF binding to these receptor sites was not competed for by a number of other growth factors which stimulate myoblast proliferation including basic fibroblast growth factor (bFGF), transforming growth factor-alpha (TGF alpha), insulin-like growth factor 1 (IGF-1), and interleukin-6 (IL-6). There was a time and concentration-dependent down-regulation of receptor numbers following preincubation of myoblasts with LIF. The processing of these receptors subsequent to binding, involves as a first step, internalization and degradation by the myoblast. LIF appeared to stimulate myoblast proliferation rather than cell survival.
机译：白血病抑制因子(生活)的一员生长因子的细胞因子家族。被证明产生的各种行为各种各样的细胞类型,包括神经元,骨,和造血细胞(1992年希尔顿,趋势物化学。类型,研究已经表明的存在生活的特定受体(戈达尔et al ., 1982年,j。Biol。Proc。国家的。尼古拉,1992年,生物。267:10238 - 10247)。受体的行为被认为涉及酪氨酸磷酸化和信号转导gp130受体组件。表明,生活能够诱导人类和小鼠成肌细胞增殖在文化(奥斯汀et al ., 1992, j .神经。112:185 - 191)。特别是表面受体成肌细胞,平衡解离常数400点和受体的数量每细胞随细胞密度。研究表明,生活结合的竞争这些受体网站没有争夺的其他一些生长因子刺激成肌细胞增殖包括基本的纤维母细胞生长因子(bFGF),将增长α因子α(TGF)、胰岛素样生长因子1 (igf - 1)、白细胞介素- 6 (il - 6)。是一个时间和浓度下调受体数量预培养成肌细胞的生活。这些受体后的处理绑定,包括作为第一步,成肌细胞内化和退化。生活似乎刺激成肌细胞增殖而不是细胞生存。

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