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首页> 外文期刊>Blood: The Journal of the American Society of Hematology >Chronic myeloid leukemia: a prospective comparison of interphase fluorescence in situ hybridization and chromosome banding analysis for the definition of complete cytogenetic response: a study of the GIMEMA CML WP.
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Chronic myeloid leukemia: a prospective comparison of interphase fluorescence in situ hybridization and chromosome banding analysis for the definition of complete cytogenetic response: a study of the GIMEMA CML WP.

机译：慢性粒细胞白血病：前期相间荧光原位杂交与染色体条带分析对完全细胞遗传反应的定义的前瞻性比较：GIMEMA CML WP的研究。

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摘要

In chronic myeloid leukemia, different methods are available to monitor the response to therapy: chromosome banding analysis (CBA), interphase fluorescence in situ hybridization (I-FISH), and real-time quantitative polymerase chain reaction (RT-Q-PCR). The GIMEMA CML WP (Gruppo Italiano Malattie Ematologiche Adulto Chronic Myeloid Leukemia Working Party) has performed a prospective study to compare CBA and I-FISH for the definition of complete cytogenetic response (CCgR). Samples (n = 664) were evaluated simultaneously by CBA and I-FISH. Of 537 cases in CCgR, the number of positive nuclei by I-FISH was less than 1% in 444 cases (82.7%). Of 451 cases with less than 1% positive nuclei by I-FISH, 444 (98.4%) were classified as CCgR by CBA. The major molecular response rate was significantly greater in cases with I-FISH less than 1% than in those with I-FISH 1% to 5% (66.8% vs 51.6%, P < .001) and in cases with CCgR and I-FISH less than 1% than in cases with CCgR and I-FISH 1% to 5% (66.1% vs 49.4%, P = .004). I-FISH is more sensitive than CBA and can be used to monitor CCgR. With appropriate probes, the cutoff value of I-FISH may be established at 1%. These trials are registered at http://www.clinicaltrials.gov as NCT00514488 and NCT00510926.
机译：在慢性粒细胞白血病中，有多种方法可用于监测对治疗的反应：染色体条带分析（CBA），相间荧光原位杂交（I-FISH）和实时定量聚合酶链反应（RT-Q-PCR）。 GIMEMA CML WP（Gruppo Italiano Malattie Ematologiche Adulto慢性骨髓性白血病工作组）进行了一项前瞻性研究，比较了CBA和I-FISH对完全细胞遗传学反应（CCgR）的定义。通过CBA和I-FISH同时评估样品（n = 664）。在537例CCgR中，I-FISH阳性核数在444例中少于1％（82.7％）。通过I-FISH检测的451例阳性核少于1％的病例中，有444例（98.4％）被CBA分类为CCgR。 I-FISH小于1％的患者的主要分子应答率明显高于I-FISH 1％至5％的患者（66.8％vs 51.6％，P <.001）以及CCgR和I-与CCgR和I-FISH病例相比，FISH小于1％，I-FISH病例为1％至5％（66.1％对49.4％，P = .004）。 I-FISH比CBA敏感，可用于监视CCgR。使用适当的探针，可以将I-FISH的截止值确定为1％。这些试验已在http://www.clinicaltrials.gov上注册为NCT00514488和NCT00510926。

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