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Cloning and Characterization of an Abalone (Haliotis discus hannai) Actin Gene

机译:鲍鱼的克隆和表征(石决明皱纹)肌动蛋白基因

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摘要

An actin encoding gene was cloned by using RT-PCR, 3' RACE and 5' RACE from abalone Haliotis discus hannai. The full length of the gene is 1532 base pairs, which contains a long 3' untranslated region of 307 base pairs and 79 base pairs of 5' untranslated sequence. The open reading frame encodes 376 amino acid residues. Sequence comparison with those of human and other mollusks showed high conservation among species at amino acid level. The identities was 96% , 97% and 96% respectively compared with Aplysia californica, Biomphalaria glabrata and Homo sapience β-actin. It is also indicated that this actin is more similar to the human cytoplasmic actin (β-actin) than to human muscle actin.
机译:一个肌动蛋白编码基因克隆利用rt - pcr,种族和5 ' 3 '从鲍鱼石决明铁饼比赛皱纹。对,其中包含3 '非翻译地区的307个碱基对,79个碱基对的5 '翻译序列。编码376个氨基酸残基。比较与人类和其他软体动物显示高保护物种间氨基酸水平。分别与海兔californica相比,Biomphalaria glabrata和人类智慧β肌动蛋白。它也表明,肌动蛋白是更多类似于人类细胞质肌动蛋白(β肌动蛋白)比人类肌肉肌动蛋白。

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