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首页> 外文期刊>Israel journal of chemistry >Bringing out the Potential of Wild-type Cytochrome P450s using Decoy Molecules: Oxygenation of Nonnative Substrates by Bacterial Cytochrome P450s
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Bringing out the Potential of Wild-type Cytochrome P450s using Decoy Molecules: Oxygenation of Nonnative Substrates by Bacterial Cytochrome P450s

机译：将野生型细胞色素的潜力使用诱饵分子p450酶类:氧化外来细菌细胞色素的基质p450酶类

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摘要

To bring out the potential of wild-type cytochrome P450s, we have developed a series of "decoy molecules" to change their high substrate specificity without any mutagenesis. Decoy molecules are inert dummy substrates with structures that are very similar to those of natural substrates. The decoy molecules force long-alkyl-chain fatty acid hydroxylases (P450(BS beta), P450(SP alpha), and P450BM3) to generate the active species and to catalyze oxidation of various sub-strates other than fatty acids. Interestingly, the catalytic activity was highly dependent on the structure of decoy molecules. Furthermore, the enantioselectivity of reactions catalyzed by P450(BS beta) and P450(SP alpha) was also dependent on the structure of decoy molecules. The decoy molecule system allows us to control reactions catalyzed by wild-type enzymes by designing decoy molecules.
机译：将野生型细胞色素的潜力p450,我们开发了一系列的“诱饵改变他们的高底物分子”没有任何突变特异性。分子是惰性假基质结构非常相似的自然基质。long-alkyl-chain脂肪酸羟化酶(P450 (BSαβ),P450 (SP)和P450BM3)来生成活跃的物种和催化氧化各种sub-strates脂肪酸。有趣的是,催化活性高依赖诱饵分子的结构。此外,反应的选择性β催化P450 (BS)和P450 (SPα)也依赖于结构的诱饵分子。控制由野生型酶催化的反应通过设计诱饵分子。

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