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首页> 外文期刊>Applied radiation and isotopes: including data, instrumentation and methods for use in agriculture, industry and medicine >Radiolabeling of Monoclonal Antibody B43.13 with Rhenium- 188 for Immunoradiotherapy
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Radiolabeling of Monoclonal Antibody B43.13 with Rhenium- 188 for Immunoradiotherapy

机译：hen 188放射标记单克隆抗体B43.13的免疫放射治疗

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摘要

In this study we report a novel method for direct radiolabeling of monoclonal antibody B43.13 (MAb-B43.13) with ↑(188)Re and have evaluated the product's radiochemical, biochemical, immunochemical and selected biological properties. ↑(188)Re- MAb- B43.13 was readily prepared by the addition of generator produced perrhenate to a preformulated antibody vial after an optimal amount of supplemental stannous ion, in the form of stannous tartrate, was added. The final radiolabeled product retained its biochemical purity (as determined by size-exclusion HPLC and R/NR-SDS-PAGE), its immunoreactivity (as determined by immunoassay) and presented with a typical stability (in the presence of serum and cysteine) and biodistribution (in tumored mice) profile. The evaluation of the product for immuno-radiotherapy of ovarian cancer in a clinical setting requires further studies. # 1997 Elsevier Science Ltd
机译：在这项研究中，我们报告了一种使用↑（188）Re直接放射性标记单克隆抗体B43.13（MAb-B43.13）的新方法，并评估了该产品的放射化学，生化，免疫化学和选定的生物学特性。 ↑（188）Re-MAb-B43.13可以通过在添加最适量酒石酸亚锡形式的最佳补充亚锡离子后，将生成器产生的高generator酸盐添加到预先配制的抗体小瓶中来轻松制备。最终的放射性标记产物保留其生化纯度（通过尺寸排阻HPLC和R / NR-SDS-PAGE测定），免疫反应性（通过免疫测定法确定）并具有典型的稳定性（在血清和半胱氨酸存在下）和生物分布（在肿瘤小鼠中）概况。在临床环境中对该产品进行卵巢癌免疫放射治疗的评估需要进一步的研究。 ＃1997爱思唯尔科学有限公司

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