• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Quality Assurance and Safety of Crops & Foods >Patulin risk associated with blue mould of pome fruit marketed in southern Italy
【24h】

Patulin risk associated with blue mould of pome fruit marketed in southern Italy

机译：棒曲霉素与蓝色梨果模具有关的风险水果市场在意大利南部

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Blue mould is one of the most important postharvest diseases of pome fruit in all producing countries. It is mainly associated to Penicillium expansum that produces the mycotoxin patulin, although other species might be involved. The aim of the present study was to characterise Penicillium isolates associated with blue mould decay of pome fruit marketed in Apulia region (southern Italy), and verify their ability to produce patulin in vitro. Twenty-nine isolates of Penicillium spp. were recovered from pome fruit showing visible blue mould symptoms, and analysed for patulin production. After fungal isolation, the fruits were singularly analysed for patulin content. In general, the isolates proved to produce patulin and most of the pome fruit contained significant amounts of patulin, but there was no quantitative correspondence between in vitro and in vivo toxin accumulation. Isolate identification at species level was based on DNA analysis by P. expansum species-specific primers and sequencing of beta-tubulin gene. Furthermore, fungal isolates were tested for the occurrence of the patN gene coding the enzyme isoepoxydon dehydrogenase (IDH), involved in patulin metabolic pathway and considered a useful indicator of critical control points for patulin contamination. All 26 isolates identified as P. expansum were positive for patN and produced patulin. Moreover, three pear isolates belonging to other Penicillium species were found. They were positive for patN, but only two actually produced patulin. It can be concluded that toxigenic P. expansum isolates are associated with blue mould of pome fruit marketed in Apulia, thus a rapid detection is important to avoid patulin contamination beyond regulatory limits. Nevertheless, the presence of patN gene alone cannot be considered a predictive assay for patulin production. An evaluation of its expression level should be carried out.
机译：蓝色的模具是最重要的之一梨果水果采后病害生产国家。青霉菌expansum产生霉菌毒素棒曲霉素,尽管其他物种参与。描述青霉菌隔离联系在一起阿普利亚蓝霉腐烂梨果水果市场意大利南部地区()和验证他们的能力棒曲霉素体外。特异种虫害的从梨果中恢复过来果显示可见的蓝色模具症状,对棒曲霉素生产分析。隔离,水果是异常分析棒曲霉素含量。证明生产棒曲霉素和大部分的梨果水果中含有大量的棒曲霉素,但是没有定量的对应关系在体外和体内毒素积累。分离鉴定物种水平DNA分析,p . expansum种群引物和beta-tubulin基因测序。此外,真菌分离株进行测试的发生patN基因编码的酶isoepoxydon脱氢酶(IDH)参与展青霉素代谢途径和被认为是有用的指标的棒曲霉素的关键控制点污染。expansum patN阳性和生产棒曲霉素。其他青霉菌物种被发现。patN是积极的,但实际上只有两个棒曲霉素生产。产毒素的p . expansum隔离相关联蓝色在阿普利亚模具梨果水果市场,因此快速检测是很重要的棒曲霉素污染超出监管限制。然而,独自patN基因的存在不能被认为是预测分析棒曲霉素产量。表达水平应该进行。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号