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Confirmation of gluten-free status of wheatgrass (Triticum aestivum)

机译:确认无谷蛋白麦草的地位(小麦)

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Young wheat (Triticum aestivum) leaves contain concentrated nutrients that may be beneficial as a nutritional supplement to those with malabsorption disorders such as celiac disease (CD) if both gluten-free, and free of gluten contamination. The tender 8-10 day leaves are juiced and the juice is consumed, added to smoothies, or consumed as a purchased wheatgrass dietary supplement in tablet, capsule and liquid forms. CD and other gluten related disorders cause both malabsorption of nutrients and an abnormal immune reaction to gluten. The only effective therapy is a lifelong gluten-free diet. Codex Alimentarius sets the gluten threshold level at 20 mg/ kg for gluten-free foods. This threshold has been adopted by many regulatory agencies such as European Commission and the US Food and Drug Administration. Consumption of wheatgrass extract has been reported to alleviate symptoms of ulcerative colitis and anaemia which are often symptoms of CD. This investigation utilised the R5 and G12 sandwich enzyme-linked immuno sorbent assays (ELISAs) to examine the potential gluten content of commercial and homegrown wheatgrass. Samples were spiked with commercial gliadin to assess recovery rates. Acceptable recovery rates ranging from 91 to 133% from gluten spiked samples were found. In all preparations the gluten content of wheatgrass leaf tissue was lower than the limit of detection and limit of quantification for both ELISAs as well as the thresholds defined by Codex Alimentarius standards. According to results presented here appropriately prepared wheatgrass contains no gluten and may have a role as a dietary supplement in a gluten-free diet.
机译:年轻的小麦(小麦)叶子包含浓缩营养物质可能是有益的患者的营养补充品吸收不良乳糜泻等疾病(CD)如果两个无谷蛋白,无麸质污染。喝醉的果汁消费,增加冰沙,或使用作为一个购买麦草膳食补充剂在平板电脑,胶囊和液体形式。导致营养吸收不良和谷蛋白异常免疫反应。有效的治疗是一个终生的无谷蛋白饮食。食品法典委员会设置面筋阈值水平在20毫克/公斤无谷蛋白食物。阈值已经通过许多监管机构,如欧洲委员会和美国食品和药物管理局。麦草提取物已经缓解溃疡性结肠炎的症状和贫血通常的症状CD。这个调查吗利用R5和G12夹心酶联免疫吸附剂测定(elisa)检测潜在的商业和面筋含量本土麦草。商业麦胶蛋白回收率进行评估。可接受的回收率从133%到91不等从面筋飙升样本被发现。准备麦草的面筋含量叶组织低于检测极限并限制的量化elisa食典委定义的阈值规范标准。这里给出适当准备麦草不含麸质,可能有一个角色无谷蛋白饮食的膳食补充剂。

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