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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Three-dimensional cellular spheroid formation provides human prostate tumor cells with tissue-like features.
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Three-dimensional cellular spheroid formation provides human prostate tumor cells with tissue-like features.

机译：三维细胞球体形成为人类前列腺肿瘤细胞提供了类似组织的特征。

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BACKGROUND: Alterations in the cellular biological responses were examined in a series of LNCaP human prostate tumor cells growing under different conditions. Materials and METHODS: LNCaP cells were grown in two-dimensional monolayer cultures, three-dimensional spheroids, or as solid tumors in immune-deprived mice. RESULTS: As compared with the growth in the monolayers, cell growth in the spheroids was reduced, while VEGF production was increased. Immunohistochemical analysis of the spheroids revealed that cells showing Ki-67 up-regulation were localized in the peripheral layer, and that the central core was necrotic. The gene expression profile in the solid tumor tissue was obviously different from that in the monolayers; however, it was similar to that in the spheroids. The prostate-specific antigen levels in the culture supernatants of spheroids increased with time and decreased with anticancer agent treatment. CONCLUSION: Spheroid formation from human prostate tumor cells exhibits tissue-like features.
机译：背景：研究了在不同条件下生长的一系列LNCaP人前列腺肿瘤细胞中细胞生物学反应的变化。材料和方法：LNCaP细胞在二维单层培养物，三维球体中生长，或在免疫剥夺的小鼠中作为实体瘤生长。结果：与单层细胞生长相比，球状细胞的细胞生长减少，而VEGF的产生增加。球体的免疫组织化学分析显示，显示Ki-67上调的细胞位于外周层，而中心核坏死。实体瘤组织中的基因表达谱明显不同于单层。但是，它类似于椭球体。球体培养上清液中的前列腺特异性抗原水平随时间增加而随抗癌剂治疗而降低。结论：人前列腺肿瘤细胞形成的球状体具有组织样特征。

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