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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Measuring cytotoxicity: a new perspective on LC50.
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Measuring cytotoxicity: a new perspective on LC50.

机译：测量细胞毒性：LC50的新观点。

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摘要

BACKGROUND: Cytotoxicity in cell culture is typically expressed as LC50 the concentration of a given agent which is lethal to 50% of the cells. This number is dependent on the incubation time with the agent. Previously, a two-term exponential model has been proposed to describe cell growth after a cytotoxic event: y(t) = k, * exp(-d(1)*t) + k2 * exp(d(2)*t). A dose-response relationship was observed between the parameter k2 in this formula and the concentration of the cytotoxic agent etoposide, in high-grade glioma cells was independent of incubation time. MATERIALS AND METHODS: In order to test if the model can be applied more generally, DAOY medulloblastoma cells treated with MS275, a histone deacetylase inhibitor, were used. RESULTS: The observed data fit the model well. CONCLUSION: The concentration at which k2 is reduced by 50% is called KC50. It provides a far better description of the cytotoxicity of an agent in a specific cell line than the traditional LC50.
机译：背景：细胞培养中的细胞毒性通常表示为对细胞50％致死的给定浓度的LC50。该数字取决于与试剂的孵育时间。以前，已经提出了两项​​指数模型来描述细胞毒性事件后的细胞生长：y（t）= k，* exp（-d（1）* t）+ k2 * exp（d（2）* t） 。在高级胶质瘤细胞中，在该配方中的参数k2与细胞毒剂依托泊苷的浓度之间观察到剂量-反应关系，而与孵育时间无关。材料和方法：为了测试该模型是否可以更广泛地应用，使用了经组蛋白脱乙酰基酶抑制剂MS275处理的DAOY髓母细胞瘤细胞。结果：观察到的数据很好地拟合了模型。结论：k2降低50％的浓度称为KC50。与传统的LC50相比，它在特定细胞系中对试剂的细胞毒性提供了更好的描述。

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