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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Comparative cytotoxicity and ROS generation by curcumin and tetrahydrocurcumin following visible-light irradiation or treatment with horseradish peroxidase.
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Comparative cytotoxicity and ROS generation by curcumin and tetrahydrocurcumin following visible-light irradiation or treatment with horseradish peroxidase.

机译：在可见光照射或用辣根过氧化物酶处理后，姜黄素和四氢姜黄素具有比较的细胞毒性和活性氧生成。

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摘要

In order to clarify the cytotoxic mechanism of curcumin, a well-known chemopreventive agent, the cytotoxicity (by MTT method), intracellular glutathione (using GSH detection kit) and intracellular reactive oxygen species (ROS) levels (with a flow cytometer), were measured in curcumin- and tetrahydrocurcumin (TH-curcumin)-treated cancer (HSG) and normal (HGF) cells under two different oxidation conditions: irradiation with visible light (VL) and enzymatic oxidation with horseradish peroxidase (HRP)/H2O2. The cytotoxicity of curcumin was highly enhanced by VL-irradiation, whereas that of TH-curcumin was enhanced by HRP/H2O2 treatment. The cytotoxicity of curcumin against HGF cells was greater than that against HSG cells. Curcumin significantly reduced the intracellular GSH level significantly under VL-irradiation, and increased it under HRP/H2O2, whereas TH-curcumin had no effect with either oxidation treatment. HRP/H2O2 treatment of TH-curcumin enhanced generation of ROS; in contrast, VL-irradiation of curcumin was considered to produce ROS preferably. In conclusion, curcumin was highly photo-toxic, caused a decrease in GSH and mediated ROS generation. In contrast, the cytotoxicity of TH-curcumin was enhanced by enzymatic oxidation. A low-level pro-oxidant intracellular milieu induced by TH-curcumin could be effectively useful for cancer prevention.
机译：为了阐明姜黄素的细胞毒性机理，使用了众所周知的化学预防剂，细胞毒性（通过MTT法），细胞内谷胱甘肽（使用GSH检测试剂盒）和细胞内活性氧（ROS）水平（使用流式细胞仪）。在两种不同的氧化条件下，用姜黄素和四氢姜黄素（TH-姜黄素）处理的癌细胞（HSG）和正常（HGF）细胞中检测到：可见光（VL）照射和辣根过氧化物酶（HRP）/ H2O2酶促氧化。 VL照射可大大增强姜黄素的细胞毒性，而HRP / H2O2处理可增强TH姜黄素的细胞毒性。姜黄素对HGF细胞的细胞毒性大于对HSG细胞的细胞毒性。姜黄素在VL辐照下显着降低了细胞内GSH的水平，而在HRP / H2O2下显着降低了其内GSH的水平，而TH-姜黄素在两种氧化处理下均没有作用。 HRP / H2O2处理TH-姜黄素可增强ROS的生成;相反，认为姜黄素的VL照射优选产生ROS。总之，姜黄素具有很高的光毒性，可导致GSH降低并介导ROS生成。相反，酶促氧化可增强TH-姜黄素的细胞毒性。 TH-姜黄素诱导的低水平促氧化剂细胞内环境可以有效地预防癌症。

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