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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Morin inhibits the growth of human leukemia HL-60 cells via cell cycle arrest and induction of apoptosis through mitochondria dependent pathway.
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Morin inhibits the growth of human leukemia HL-60 cells via cell cycle arrest and induction of apoptosis through mitochondria dependent pathway.

机译：Morin通过细胞周期停滞和通过线粒体依赖性途径诱导细胞凋亡来抑制人白血病HL-60细胞的生长。

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摘要

The effects of morin (3,5,7,20,40-pentahydroxyflavone) on human leukemia HL-60 cells in vitro were investigated and the molecular mechanisms of morin-induced G2/M arrest and apoptosis in HL-60 cells were examined. Morin induced morphological changes and decreased the percentage of viable cells via induction of G2/M-phase arrest and apoptosis. Morin-induced G2/M-phase arrest was accompanied by the promotion of p21 and Wee1, and decreased levels of Cdc25c and cyclins A and B1 complex. Morin-induced apoptosis in HL-60 cells was also confirmed by flow cytometric assay, DNA gel electrophoresis for DNA fragmentation and DAPI staining. Morin induced apoptosis in time- and dose-dependent manners. Morin-induced apoptosis was associated with elevated intracellular reactive oxygen species (ROS) increased and Ca2+ production; decreased the levels of mitochondria membrane potential (deltapsi(m)) and increased caspase-3 activation. Collectively, these results suggest that the morin-induced apoptosis in HL-60 cells may result from the activation of caspase-3 and intracellular Ca2+ release, as well as the mitochondria membrane potential pathway.
机译：研究了茉莉酸（3,5,7,20,40-五羟基黄酮）对人白血病HL-60细胞的影响，并研究了茉莉酸诱导HL-60细胞G2 / M阻滞和凋亡的分子机制。桑色素通过诱导G2 / M期阻滞和凋亡而诱导形态变化并降低了存活细胞的百分比。 Morin诱导的G2 / M期阻滞伴随着p21和Wee1的促进，以及Cdc25c和cyclins A和B1复合物水平的降低。流式细胞术，DNA凝胶电泳，DNA片段化和DAPI染色也证实了Morin诱导的HL-60细胞凋亡。桑色素以时间和剂量依赖性方式诱导细胞凋亡。 Morin诱导的细胞凋亡与细胞内活性氧（ROS）升高和Ca2 +产生相关。降低线粒体膜电位水平（deltapsi（m））和增加caspase-3激活。总的来说，这些结果表明，由林蛋白诱导的HL-60细胞凋亡可能是由caspase-3的激活和细胞内Ca2 +的释放以及线粒体膜电位途径引起的。

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