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首页> 外文期刊>Applied Spectroscopy: Society for Applied Spectroscopy >Analysis of Reflectance and Transmittance Measurements on Absorbing and Scattering Small Samples Using a Modified Integrating Sphere Setup
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Analysis of Reflectance and Transmittance Measurements on Absorbing and Scattering Small Samples Using a Modified Integrating Sphere Setup

机译：使用改进的积分球设置分析吸收和散射小样品的反射率和透射率

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摘要

The aim of the present paper is to analyze reflectance and transmittance measurements on small scattering and absorbing samples. The long term goal is to perform quantitative, spectroscopic in vivo measurements of pigments in small samples of plant material. Small samples such as small leaves constitute a special experimental challenge in cases in which the sample beam has a larger cross-sectional area than the sample. The experimental errors introduced when measuring reflectance and transmittance on small absorbing and scattering samples are investigated theoretically and experimentally by using a blue polyester sample as an appropriate test sample. The experiments are performed with either a mask or a lens setup combined with a mask inserted in the sample beam. In particular, the errors introduced in the reflectance measurements can be very large and larger than 100percent. It is shown that any direct illumination of the mask must be avoided. To obtain more accurate values for the reflection coefficient it is necessary to combine the mask with a focusing lens system, adjust the mask and sample very carefully, and choose the ratio between the aperture of the mask and the beam area as large as possible. In the case of transmittance measurements, it is shown that the combination of a special sample fixture and a lens system gives rise to smaller errors compared to the errors introduced by the mask alone or the mask combined with a focusing lens system.
机译：本文的目的是分析小散射和吸收样品的反射率和透射率测量。长期目标是对少量植物材料样品中的色素进行定量，光谱体内测量。在样品光束的横截面面积大于样品横截面的情况下，小样品（例如小叶子）构成了特殊的实验挑战。通过使用蓝色聚酯样品作为适当的测试样品，从理论上和实验上研究了在测量小的吸收和散射样品上的反射率和透射率时引入的实验误差。实验是通过将掩模或透镜装置与插入样品光束中的掩模相结合来进行的。特别是，反射率测量中引入的误差可能非常大，甚至大于100％。结果表明，必须避免直接照射面罩。为了获得更准确的反射系数值，必须将光罩与聚焦透镜系统结合在一起，非常仔细地调整光罩和样品，并选择光罩孔径与光束面积之间的比例尽可能大。在透射率测量的情况下，显示出与单独的掩模或与聚焦透镜系统结合的掩模所引入的误差相比，特殊的样品夹具和透镜系统的组合产生的误差较小。

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