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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Physicochemical conditions affecting the formation/stability of serum complexes and the determination of prostate-specific antigen (PSA).
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Physicochemical conditions affecting the formation/stability of serum complexes and the determination of prostate-specific antigen (PSA).

机译：影响血清复合物形成/稳定性和前列腺特异性抗原（PSA）测定的理化条件。

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摘要

Inaccurate determination of total prostate-specific antigen (t-PSA) mainly derives from inadequate estimation of this heterogeneous molecule and its complexes with serum protease inhibitors, such as the a,-antichymotrypsin (ACT) and alpha 2-macroglobulin (alpha 2M). While ACT-PSA complex is confirmed to be an important quantity for prostate cancer (PCa) diagnosis, alpha 2M-PSA complex is still an unmeasurable fraction. This study was performed to evaluate the phycicochemical conditions of PSA complex formation with serum protease inhibitors, focusing on ACT and alpha 2M. t-PSA and free prostate-specific antigen (f-PSA) levels were estimated with commercial immunoassays (Axsym/Abbott, Enzymun/Boehringer Mannheim, Elfa/ Biomerieux), while the formation of PSA complexes with ACT and alpha 2M with Western-blot electrophoresis. t-PSA values were unexpectedly lower after incubation of semen PSA for 4 days at 4 degrees C or at 37 degrees C in female serum and goat serum relevant to incubation in BSA buffer, possibly due to immunoreactivity loss in alpha 2M-PSA formation in the serum matrices. The transformation of PSA molecule into various measured and unmeasured forms after exposure to serum, especially suggested the inadequacy of serum matrices for f-PSA standard preparation. Loss of stability in PSA complexes was observed after dilution of prostate cancer (PCa) serum in aqueous buffer (BSA buffer), possibly due to dissociation of complex structure, the effect being mended by prior excess addition of ACT or alpha 2M. Optimum pH (approximately 7.5) and temperature (37 degrees C) for serum protease inhibitors binding to PSA were these of human serum, the complex formation increasing with incubation time, but not with PSA concentration.
机译：总前列腺特异性抗原（t-PSA）的不准确测定主要是由于对该异质分子及其与血清蛋白酶抑制剂（例如α-抗胰凝乳蛋白酶（ACT）和α2-巨球蛋白（alpha 2M））的复合物的估计不足。虽然ACT-PSA复合物已被证实是诊断前列腺癌（PCa）的重要量，但α2M-PSA复合物仍是不可测量的部分。进行这项研究以评估与血清蛋白酶抑制剂形成PSA复合物的理化条件，重点是ACT和alpha 2M。通过商业免疫分析（Axsym / Abbott，Enzymun / Boehringer Mannheim，Elfa / Biomerieux）评估了t-PSA和游离前列腺特异性抗原（f-PSA）的水平，同时形成了带有ACT的PSA复合物和带有Western-blot的alpha 2M电泳。与在BSA缓冲液中孵育有关的雌性血清和山羊血清中的精液PSA在4°C或37°C下孵育4天后，t-PSA值出乎意料地降低，这可能是由于在小鼠血清中α2M-PSA形成中的免疫反应性丧失。血清基质。暴露于血清后，PSA分子转化为各种可测量和不可测量的形式，尤其表明f-PSA标准制剂的血清基质不足。在水性缓冲液（BSA缓冲液）中稀释前列腺癌（PCa）血清后，观察到PSA复合物的稳定性下降，这可能是由于复合物结构的解离所致，这种作用可通过事先过量添加ACT或alpha 2M来弥补。结合PSA的血清蛋白酶抑制剂的最佳pH（约7.5）和温度（37℃）为人血清，复合物的形成随孵育时间的增加而增加，但不随PSA浓度的增加而增加。

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