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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Nanodiamond and nanoplatinum liquid, DPV576, activates human monocyte-derived dendritic cells in vitro.
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Nanodiamond and nanoplatinum liquid, DPV576, activates human monocyte-derived dendritic cells in vitro.

机译：纳米金刚石和纳米铂液体DPV576在体外激活人单核细胞衍生的树突状细胞。

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BACKGROUND: The influence of nanoparticles on the immune system is poorly understood. It was recently shown that exposure to a mixture of nanodiamond (ND)- and nanoplatinum (NP)-coated material (DPV576-C) activates murine T-cells. This study examined the role of a dispersed aqueous mixture of ND/NP (DPV576) in activating human dendritic cells (DCs) in vitro. MATERIALS AND METHODS: Human monocyte-derived DCs were treated with DPV576 at various concentrations (50, 100 and 200 mug/ml) for 24 hours in vitro. Activation of DCs was determined by assessing the expression of co-stimulatory and maturation markers (CD80, CD83, CD86, HLADR), production of cytokines, and induction of proliferation of naive CD4 T-cells. Expression of co-stimulatory molecules and cell proliferation were analysed by flow cytometry and cytokine secretion by ELISA. RESULTS: DPV576 treatment of DCs resulted in: (i) increased CD83 and CD86 expression on DCs, (ii) up-regulation in the levels of DC-secreted cytokines IL-6, TNF and IL-10, and (iii) increased ability to induce proliferation in CD4(+) T-cells which is associated with increased expression of T-cell activation marker CD25. CONCLUSION: Solution containing ND/NP (DPV576) activated human DCs and DCs-driven CD4 naive T-cell proliferation in vitro, which may be useful in boosting immune responses in cancer treatment.
机译：背景：纳米粒子对免疫系统的影响知之甚少。最近显示，暴露于纳米金刚石（ND）和纳米铂（NP）涂层材料（DPV576-C）的混合物中会激活鼠T细胞。这项研究检查了ND / NP（DPV576）的分散水混合物在体外激活人树突状细胞（DC）中的作用。材料与方法：将人单核细胞衍生的DC用DPV576于各种浓度（50、100和200杯/毫升）进行体外处理24小时。通过评估共刺激和成熟标志物（CD80，CD83，CD86，HLADR）的表达，细胞因子的产生以及诱导天然CD4 T细胞的增殖来确定DC的激活。通过流式细胞术分析共刺激分子的表达和细胞增殖，并通过ELISA分析细胞因子的分泌。结果：DPV576对DC的治疗导致：（i）DC上CD83和CD86表达增加，（ii）DC分泌的细胞因子IL-6，TNF和IL-10的水平上调，以及（iii）能力增强诱导CD4（+）T细胞增殖，这与T细胞活化标记CD25的表达增加有关。结论：含有ND / NP（DPV576）激活的人DC和DCs驱动的CD4幼稚T细胞增殖的体外溶液，可能有助于增强癌症治疗中的免疫应答。

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