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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >HAVcR-1 reduces the integrity of human endothelial tight junctions.
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HAVcR-1 reduces the integrity of human endothelial tight junctions.

机译：HAVcR-1降低了人内皮紧密连接的完整性。

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摘要

BACKGROUND: Hepatitis A virus cellular receptor-1 (HAVcR-1) is the cellular receptor for Hepatotropic picornavirus. Although HAVcR-1 is expressed in every human organ, its natural function remains unknown. This study investigated the location, association and functionality of HAVcR-1 in human endothelial cells. MATERIALS AND METHODS: HAVcR-1 was either overexpressed or knocked-down by plasmid electroporation in human umbilical vein endothelial (HECV) cells. Changes in tight junction (TJ) behaviour were assessed using transendothelial resistance, and paracellular permeability. Binding partners and the location of HAVcR-1 protein was assessed using Western blotting/immunofluorescence. RESULTS: HAVcR-1 co-localised with zonula occludens-1 (ZO-1) and ZO-2 proteins, both of which are involved in the formation, maintenance and function of TJ. The overexpression of HAVcR-1 resulted in reduced TJ formation; knockdown cells were resistant to hepatocyte growth factor (HGF)-mediated TJ disruption. HGF was unable to effect reduced resistance in these cells. HAVcR-1 was co-precipitated with the TJ regulatory factor Ras homolog gene family, member C (Rho C). CONCLUSION: HAVcR-1 may have a novel function as part of the regulatory apparatus for TJ in human endothelial cells.
机译：背景：甲型肝炎病毒细胞受体-1（HAVcR-1）是嗜肝性小核糖核酸病毒的细胞受体。尽管HAVcR-1在每个人体器官中都有表达，但其天然功能仍然未知。这项研究调查了人类内皮细胞中HAVcR-1的位置，关联和功能。材料与方法：在人脐静脉内皮细胞（HECV）中通过质粒电穿孔法过表达HAVcR-1或敲低HAVcR-1。使用跨内皮电阻和细胞旁通透性评估紧密连接（TJ）行为的变化。使用蛋白质印迹/免疫荧光评估结合伴侣和HAVcR-1蛋白的位置。结果：HAVcR-1与闭合小带1（ZO-1）和ZO-2蛋白共定位，两者均参与TJ的形成，维持和功能。 HAVcR-1的过表达导致TJ形成减少;击倒细胞对肝细胞生长因子（HGF）介导的TJ破坏有抵抗力。 HGF无法在这些细胞中降低耐药性。 HAVcR-1与TJ调节因子Ras同源基因家族成员C（Rho C）共沉淀。结论：HAVcR-1可能是人内皮细胞中TJ调节装置的一部分。

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