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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Ruta graveolens extract induces DNA damage pathways and blocks Akt activation to inhibit cancer cell proliferation and survival.
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Ruta graveolens extract induces DNA damage pathways and blocks Akt activation to inhibit cancer cell proliferation and survival.

机译：芸苔提取物诱导DNA损伤途径并阻断Akt活化，从而抑制癌细胞的增殖和存活。

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摘要

BACKGROUND: Ruta graveolens is a medicinal herb that has been used for centuries against various ailments. This study examined the anticancer properties of the herb using cancer cell lines. MATERIALS AND METHODS: Methanolic extract of R. graveolens was tested on colon, breast and prostate cancer cells. Viability, cell cycle profiles, clonogenicity and capase activation were measured. Induction and subcellular localizations of p53, 53BP1 and gamma-H2AX proteins were examined. RESULTS: The extract dose-dependently decreased the viability and the clonogenicity of treated cells and induced G/M arrest, aberrant mitoses, and caspase-3 activation. It also induced the p53 pathway and focal concentration of the DNA damage response proteins 53BP1 and gamma-H2AX. Moreover, the levels of phospho-Akt and cyclin B1 were reduced by treatment, whereas only cyclin B1 was reduced in normal dermal fibroblasts. CONCLUSION: R. graveolens extract contains bioactive compounds which, independently of known photoactivatable mechanisms, potently inhibit cancer cell proliferation and survival through multiple targets.
机译：背景：芸香（Rutagravolens）是一种已经使用了数百年的抗各种疾病的草药。这项研究使用癌细胞系检查了草药的抗癌特性。材料和方法：在结肠癌，乳腺癌和前列腺癌细胞上测试了R.gravolens的甲醇提取物。测量了活力，细胞周期概况，克隆形成性和capase活化。检查了p53、53BP1和γ-H2AX蛋白的诱导和亚细胞定位。结果：提取物剂量依赖性地降低了被处理细胞的活力和克隆形成能力，并诱导了G / M阻滞，有丝分裂和caspase-3活化。它还诱导了DNA损伤反应蛋白53BP1和gamma-H2AX的p53途径和聚焦浓度。而且，通过治疗降低了磷酸化Akt和细胞周期蛋白B1的水平，而在正常的皮肤成纤维细胞中仅细胞周期蛋白B1降低了。结论：鼠李糖提取物含有生物活性化合物，其与已知的光激活机制无关，可通过多个靶点有效抑制癌细胞的增殖和存活。

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