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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein
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The structure of the C-terminal domain of the Zaire ebolavirus nucleoprotein

机译：的c端域的结构扎伊尔ebolavirus核蛋白质

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摘要

Ebolavirus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation. NP is unusual among the Mononegavirales in that it contains two distinct regions, or putative domains, the C-terminal of which shows no homology to any known proteins and is purported to be a hub for protein-protein interactions within the nucleocapsid. The atomic structure of NP remains unknown. Here, the boundaries of the N- and C-terminal domains of NP from Zaire EBOV are defined, it is shown that they can be expressed as highly stable recombinant proteins in Escherichia coli, and the atomic structure of the C-terminal domain (residues 641-739) derived from analysis of two distinct crystal forms at 1.98 and 1.75 angstrom resolution is described. The structure reveals a novel tertiary fold that is distantly reminiscent of the beta-grasp architecture.
机译：Ebolavirus (EBOV)会导致严重的出血热死亡率高达90%。订单Mononegavirales和成员其他病毒在这个分类组,包含一个消极意义上讲单链RNA (ss)。ssRNA编码七个不同的蛋白质。他们,核蛋白(NP),是最病毒蛋白在感染细胞和丰富病毒核衣壳内。蛋白NP是多功能的。与病毒基因组,是至关重要的RNA复制,转录病毒基因组包装和核衣壳组装前膜封装。Mononegavirales,它包含两个不同的地区,或假定的域,c端显示没有任何已知的蛋白质和同源性据说是一个蛋白质中心吗核衣壳内的相互作用。NP结构仍然是未知的。边界的N - NP和c端域从扎伊尔EBOV定义,它是显示他们可以表达为高度稳定重组蛋白在大肠杆菌,原子结构的c端域(641 - 739)残留来自两个分析不同的晶体形式在1.98和1.75埃解析描述。小说第三冷淡地回想起褶皱beta-grasp的架构。

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