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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Satellite tobacco mosaic virus refined to 1.4 angstrom resolution
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Satellite tobacco mosaic virus refined to 1.4 angstrom resolution

机译：1.4卫星烟草花叶病毒精制埃分辨率

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Satellite tobacco mosaic virus (STMV) is among the smallest viruses, having 60 identical subunits arranged with T = 1 icosahedral symmetry. Its crystal structure was solved at 290 K and was refined using, in part, crystals grown in microgravity. Electron-density maps revealed nearly 57% of the genomic ssRNA. Using six flash-cooled crystals, diffraction data were recorded to 1.4 angstrom resolution and independent refinements of the STMV model were carried out versus the previous 1.8 angstrom resolution data representing merged data from 21 crystals (271 689 unique reflections), data consisting of corresponding reflections to 1.8 angstrom resolution from the cooled crystals and 1.4 angstrom resolution data from the cooled crystals comprised of 570 721 unique reflections. Models were independently refined with full NCS constraints using the program CNS and in restrained mode using the programs CNS, REFMAC5 and SHELX-97, with the latter two procedures including anisotropic temperature factors. Significant additional structural detail emerged from the analyses, including a unique cation and anion arrangement on fivefold axes and a precise assessment of icosahedral symmetry exactness in the crystal lattice. STMV represents the highest resolution native virus structure currently known by a substantial margin, and it permits the evaluation of a precise atomic model of a spherical virus at near-atomic resolution for the first time.
机译：卫星烟草花叶病毒(STMV)就是其中之一最小的病毒,有60相同的子单元安排和T = 1二十面体对称。晶体结构解决了在290 K精制使用,在一定程度上,晶体生长在微重力环境下的骨质流失。近57%的基因组ssRNA。flash-cooled晶体衍射数据1.4埃分辨率和记录独立STMV模型的改进与之前的1.8埃从21分辨率数据代表合并数据晶体(271 689独特的反射),数据由相应的反射到1.8埃分辨率的水晶和冷却从冷却1.4埃分辨率数据晶体组成的570 721独特的反射。模型分别与完整的nc精制使用程序中枢神经系统和约束约束模式使用程序的中枢神经系统,REFMAC5和shelx - 97,后两个过程包括各向异性温度因子。大量额外结构细节浮出水面从分析,包括一个独特的阳离子和阴离子安排5倍轴和精确二十面体对称精确的评估晶格。目前已知解决本地病毒结构要高出一大截,它允许评价一个精确的原子模型球形病毒在near-atomic决议已经不是第一次了。

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