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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line.
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Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line.

机译：木质素-碳水化合物复合物从香菇菌丝体提取物（LEM）中的木质素-碳水化合物复合物在小鼠巨噬细胞样细胞系中增强Dectin-2基因表达。

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摘要

BACKGROUND: Lignin fractions of Lentinus edodes mycelia extract (LEM) have shown anti-HIV and immunopotentiating activity. However, the action point of lignin-carbohydrate complex has not been elucidated. In order to elucidate their action point, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. MATERIALS AND METHODS: RNA was isolated with Qiagen RNeasy Plus Mini kit, hydridized with GeneChip MouseGene 1.0 ST arrays, and scanned with Affymetrix GeneChip Command Console software. RESULTS: One of the seven lignin-carbohydrate fractions isolated from LEM (Fr4) enhanced the expression of dectin-2 (4.2-fold) and toll-like receptor (TLR)-2 (2.5-fold) prominently, but only slightly modified the expression of dectin-1 (0.8-fold), complement receptor 3 (0.9-fold), TLR1, 3, 4, 9 and 13 (0.8- to 1.7-fold), spleen tyrosine kinase (Syk)b, zeta-chain (TCR) associated 70 kDa protein kinase (Zap70), Janus tyrosine kinase (Jak)2 (1.0- to 1.2-fold), nuclear factor (Nf)small ka, Cyrillicb1, NFsmall ka, Cyrillicb2, reticuloendotheliosis viral oncogene homolog (Rel)a, Relb (1.0- to 1.6-fold), Nfsmall ka, Cyrillicbia, Nfsmall ka, Cyrillicbib, Nfsmall ka, Cyrillicbie, Nfsmall ka, Cyrillicbil2 and Nfsmall ka, Cyrillicbiz (0.8- to 2.3-fold). On the other hand, lipopolysaccharide did not affect the expression of dectin-2 or TLR-2. CONCLUSION: These data suggest the significant role of the activation of the dectin-2 signaling pathway in the action of lignin-carbohydrate complex on macrophages.
机译：背景：香菇菌丝体提取物（LEM）的木质素部分具有抗HIV和免疫增强作用。但是，木质素-碳水化合物复合物的作用点尚未阐明。为了阐明其作用点，使用小鼠巨噬细胞样J774.1细胞进行了DNA微阵列分析。材料与方法：用Qiagen RNeasy Plus Mini试剂盒分离RNA，用GeneChip MouseGene 1.0 ST阵列水解，并用Affymetrix GeneChip Command Console软件扫描。结果：从LEM（Fr4）分离的七个木质素碳水化合物馏分之一显着增强dectin-2（4.2倍）和toll样受体（TLR）-2（2.5倍）的表达，但仅轻微修饰dectin-1（0.8倍），补体受体3（0.9倍），TLR1、3、4、9和13（0.8-1.7倍），脾酪氨酸激酶（Syk）b，ζ链（ TCR）相关的70 kDa蛋白激酶（Zap70），Janus酪氨酸激酶（Jak）2（1.0至1.2倍），核因子（Nf）small ka，Cyrillicb1，NFNFsmall ka，Cyrillicb2，网状内皮病病毒癌基因同源物（Rel）a ，Relb（1.0-至1.6倍），Nfsmall ka，西里尔比亚，Nfsmall ka，Cyrillicbib，Nfsmall ka，西里尔比比，Nfsmall ka，Cyrillicbil2和Nfsmall ka，西里尔（0.8-至2.3倍）。另一方面，脂多糖不影响dectin-2或TLR-2的表达。结论：这些数据表明，dectin-2信号通路的激活在木质素-碳水化合物复合物对巨噬细胞的作用中具有重要作用。

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