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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression.
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Circulating Human Prostate Cancer Cells from an Orthotopic Mouse Model Rapidly Captured by Immunomagnetic Beads and Imaged by GFP Expression.

机译：循环人前列腺癌细胞从原位小鼠模型快速捕获的免疫磁珠和GFP表达成像。

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摘要

Circulating tumor cells (CTCs) are potential precursors of metastasis. They are also of use in diagnosing malignancy and for prognostic purposes. Our laboratory has previously isolated CTCs from orthotopic nude mouse models of human prostate cancer cells where the PC-3 cancer cells express green fluorescent protein (GFP). It was found that orthotopic tumors produced CTCs and not subcutaneous tumors, which may explain why orthotopic tumors metastasize and subcutaneous tumors do not. However, in this previous study, CTCs were observed only after culture. In the present study, using the GFP-expressing PC-3 orthotopic model and immunomagnetic beads coated with anti-epithelial cell adhesion molecule (EpCAM) and anti-prostate specific membrane antigen (PSMA), GFP-expressing CTC were isolated within 15 minutes and were readily visualized by GFP fluorescence. It was possible to immediately place the immunomagnetic-bead-captured GFP-expressing PC-3 CTCs in 3-dimensional sponge cell culture, where they proliferated. The combination of GFP expression and the use of immunomagnetic beads is a very powerful method to obtain CTCs for either immediate analysis or for biological characterization in vivo or in 3-dimensional culture.
机译：循环肿瘤细胞（CTC）是潜在的转移前体。它们也可用于诊断恶性肿瘤和用于预后目的。我们的实验室以前从人类前列腺癌细胞的原位裸鼠模型中分离出四氯化碳，其中PC-3癌细胞表达绿色荧光蛋白（GFP）。发现原位肿瘤产生CTC而不产生皮下肿瘤，这可以解释为什么原位肿瘤没有转移和皮下肿瘤。但是，在先前的研究中，仅在培养后才观察到四氯化碳。在本研究中，使用表达GFP的PC-3原位模型和涂有抗上皮细胞粘附分子（EpCAM）和抗前列腺特异性膜抗原（PSMA）的免疫磁珠，在15分钟内分离了表达GFP的CTC，通过GFP荧光很容易观察到。可以立即将表达免疫磁珠的表达GFP的PC-3 CTC置于3维海绵细胞培养物中，并在那里增殖。 GFP表达与免疫磁珠的结合使用是获得CTC的非常有效的方法，可用于即时分析或体内或三维培养中的生物学表征。

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