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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Modeling the SHG activities of diverse protein crystals
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Modeling the SHG activities of diverse protein crystals

机译：建模的宋惠乔活动不同的蛋白质晶体

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A symmetry-additive ab initio model for second-harmonic generation (SHG) activity of protein crystals was applied to assess the likely protein-crystal coverage of SHG microscopy. Calculations were performed for 250 proteins in nine point-group symmetries: a total of 2250 crystals. The model suggests that the crystal symmetry and the limit of detection of the instrument are expected to be the strongest predictors of coverage of the factors considered, which also included secondary-structural content and protein size. Much of the diversity in SHG activity is expected to arise primarily from the variability in the intrinsic protein response as well as the orientation within the crystal lattice. Two or more orders-of-magnitude variation in intensity are expected even within protein crystals of the same symmetry. SHG measurements of tetragonal lysozyme crystals confirmed detection, from which a protein coverage of 84% was estimated based on the proportion of proteins calculated to produce SHG responses greater than that of tetragonal lysozyme. Good agreement was observed between the measured and calculated ratios of the SHG intensity from lysozyme in tetragonal and monoclinic lattices.
机译：symmetry-additive从头计算模型二次谐波产生(宋惠乔)的活动蛋白质晶体应用于评估的可能测定晶体宋惠乔显微镜的报道。计算250年进行蛋白质9点群对称性:2250晶体。对称的检测极限预计将最强大的工具预测范围的因素考虑,包括secondary-structural内容和蛋白质的大小。主要从活动预计将出现变化的内在蛋白反应在晶体的取向晶格。强度的变化预计即使在蛋白质晶体的对称性。正方溶菌酶晶体的测量确认检测、蛋白质估计基于覆盖率84%生产宋惠乔蛋白质的比例计算反应大于正方溶菌酶。测量并计算宋惠乔的比率强度在正方和溶菌酶单斜晶体的晶格。

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