...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Two-photon excited UV fluorescence for protein crystal detection.
【24h】

Two-photon excited UV fluorescence for protein crystal detection.

机译：双光子激发紫外荧光蛋白质晶体检测。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two-photon excited ultraviolet fluorescence (TPE-UVF) microscopy is explored for sensitive protein-crystal detection as a complement to second-order nonlinear optical imaging of chiral crystals (SONICC). Like conventional ultraviolet fluorescence (UVF), TPE-UVF generates image contrast based on the intrinsic fluorescence of aromatic residues, generally producing higher fluorescence emission within crystals than the mother liquor by nature of the higher local protein concentration. However, TPE-UVF has several advantages over conventional UVF, including (i) insensitivity to optical scattering, allowing imaging in turbid matrices, (ii) direct compatibility with conventional optical plates and windows by using visible light for excitation, (iii) elimination of potentially damaging out-of-plane UV excitation, (iv) improved signal to noise through background reduction from out-of-plane excitation and (v) relatively simple integration into instrumentation developed for SONICC.
机译：双光子激发紫外荧光(TPE-UVF)显微镜探讨敏感测定晶体检测作为补充二阶非线性光学成像的手性晶体(SONICC)。荧光(UVF), TPE-UVF生成图像基于固有荧光的对比芳香的残留物,一般生产更高在晶体的荧光发射母液的自然更高的地方蛋白质的浓度。几个优势传统的紫外荧光,包括光学(我)不敏感在浑浊的散射,使成像矩阵,(2)与传统的直接兼容利用可见光光学板和窗户激发,(3)消除潜在的破坏性的平面外紫外线激发,(iv)通过背景改善信号噪声减少从平面外激励和(v)相对简单的融入针对SONICC开发的仪器。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号