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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance.
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Reduced HIC-1 gene expression in non-small cell lung cancer and its clinical significance.

机译：非小细胞肺癌中HIC-1基因表达降低及其临床意义。

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摘要

BACKGROUND: HIC-1 (hypermethylated in cancer-1) is a candidate tumor suppressor gene, identified in a region of frequent loss of heterozygosity on chromosome 17p13.3, which is telomeric from TP53 and often deleted in surgically resected lung cancers. To determine the significance of HIC-1 in lung cancer, we assessed its expression status and prognostic association in 47 adenocarcinomas and squamous cell carcinomas. MATERIALS AND METHODS: RNA was extracted from tumors and corresponding normal tissues of surgically resected lungs, and the amount of HIC-1 mRNA was determined by means of semi-quantitative reverse transcriptase-polymerase chain reaction. RESULTS: HIC-1 expression in tumors was less than that in normal lung tissues in 40 of 47 patients (85%), indicating frequent partial silencing. Median tumorormal lung tissue (T/L) ratios for HIC-1 expression were 0.51 and 0.75 for adenocarcinomas and squamous cell carcinomas, respectively. No significant difference of median T/L ratio was observed between the two histological types, or among clinical stages of the patients. However, the reduced expression of HIC-1 gene in the tumor had a direct link with the clinical outcome: lower T/L ratios (< 0.5) were significantly associated with short survival (P = 0.034), an association also observed in cases restricted to stage I (P = 0.047). CONCLUSIONS: The results suggest that low HIC-1 expression is involved in malignant progression of non-small cell lung cancer.
机译：背景：HIC-1（在癌症1中为高甲基化）是一种候选的抑癌基因，在染色体17p13.3上经常失去杂合性的区域鉴定出，该基因是TP53的端粒，在手术切除的肺癌中经常缺失。为了确定HIC-1在肺癌中的重要性，我们评估了其在47例腺癌和鳞状细胞癌中的表达状态和预后关联。材料与方法：从手术切除的肺部肿瘤及相应的正常组织中提取RNA，并通过半定量逆转录聚合酶链反应测定HIC-1 mRNA的含量。结果：47例患者中有40例（85％）的肿瘤中HIC-1表达低于正常肺组织，表明其频繁出现局部沉默。腺癌和鳞癌中HIC-1表达的中位肿瘤/正常肺组织（T / L）比分别为0.51和0.75。在两种组织学类型之间或患者的临床阶段之间，未观察到中位T / L比的显着差异。然而，肿瘤中HIC-1基因表达的降低与临床结果直接相关：较低的T / L比（<0.5）与短生存期显着相关（P = 0.034），在限制性病例中也观察到这种关系进入第一阶段（P = 0.047）。结论：结果表明低HIC-1表达与非小细胞肺癌的恶性进展有关。

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