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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Temperature-dependent macromolecular X-ray crystallography.
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Temperature-dependent macromolecular X-ray crystallography.

机译：与温度有关的大分子x射线晶体学。

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摘要

X-ray crystallography provides structural details of biological macromolecules. Whereas routine data are collected close to 100 K in order to mitigate radiation damage, more exotic temperature-controlled experiments in a broader temperature range from 15 K to room temperature can provide both dynamical and structural insights. Here, the dynamical behaviour of crystalline macromolecules and their surrounding solvent as a function of cryo-temperature is reviewed. Experimental strategies of kinetic crystallography are discussed that have allowed the generation and trapping of macromolecular intermediate states by combining reaction initiation in the crystalline state with appropriate temperature profiles. A particular focus is on recruiting X-ray-induced changes for reaction initiation, thus unveiling useful aspects of radiation damage, which otherwise has to be minimized in macromolecular crystallography.
机译：x射线晶体学提供了结构的细节的生物大分子。收集的数据是接近100 K为了减轻辐射损伤,更多的异国情调在一个更广泛的温度控制实验温度范围从15 K到室温可以提供动力和结构的见解。水晶大分子及其周围溶剂cryo-temperature的函数回顾。讨论了晶体学所允许的大分子的生成和捕获中间状态相结合的反应起始的结晶状态适当的温度资料。重点是招聘X-ray-induced变化反应起始,从而推出有用方面的辐射损伤,否则在大分子最小化晶体学。

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