Features of the secondary structure of a protein molecule from powder diffraction data.

Basso S; Besnard C; Wright JPMargiolaki IFitch APattison PSchiltz M

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Features of the secondary structure of a protein molecule from powder diffraction data.

【24h】

Features of the secondary structure of a protein molecule from powder diffraction data.

机译：蛋白质的二级结构的特性分子从粉末衍射数据。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Protein powder diffraction is shown to be suitable for obtaining de novo solutions to the phase problem at low resolution via phasing methods such as the isomorphous replacement method. Two heavy-atom derivatives (a gadolinium derivative and a holmium derivative) of the tetragonal form of hen egg-white lysozyme were crystallized at room temperature. Using synchrotron radiation, high-quality powder patterns were collected in which pH-induced anisotropic lattice-parameter changes were exploited in order to reduce the challenging and powder-specific problem of overlapping reflections. The phasing power of two heavy-atom derivatives in a multiple isomorphous replacement analysis enabled molecular structural information to be obtained up to approximately 5.3 A resolution. At such a resolution, features of the secondary structure of the lysozyme molecule can be accurately located using programs dedicated to that effect. In addition, the quoted resolution is sufficient to determine the correct hand of the heavy-atom substructure which leads to an electron-density map representing the protein molecule of proper chirality.

机译：蛋白质粉末衍射证明是合适的获取新创解决阶段通过分阶段方法在低分辨率的问题比如同形的替代方法。重原子衍生物(钆导数和钬正方的导数)的形式母鸡蛋清溶菌酶的结晶室温。高质量的粉末收集模式这pH-induced各向异性晶格参数改变是为了减少具有挑战性和powder-specific问题重叠的倒影。重原子衍生物在多个同形替代分析使分子结构信息获得的约5.3一项决议。溶菌酶的二级结构分子可以准确查找使用程序专用的。足以确定正确的决议手重原子子结构的线索电子密度图表示蛋白质分子的手性。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2010年第7期|756-761|共6页
作者
Basso S; Besnard C; Wright JPMargiolaki IFitch APattison PSchiltz M;
展开▼
作者单位

Ecole Polytechnique Federale de Lausanne (EPFL), Laboratoire de Cristallographie, Lausanne, Switzerland.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
Powder Diffraction; isomorphous substitution;

机译：粉末衍射,同形替换;

相似文献

外文文献
中文文献
专利

1. Comparative crystal structure determination of griseofulvin： Powder X-ray diffraction versus single-crystal X-ray diffraction [J] . PAN QingQing, GUO Ping, DUAN Jiong, 中国科学通报：英文版 . 2012,第030期
2. X-Ray Powder Diffraction Data—Identification and Structure Elucidation of Unknown Stone—Rietveld Refinement Approach [J] . Munusamy Baskar, Anju Patania 生物物理化学（英文） . 2020,第004期
3. {CONFORMATIONAL} {SAMPLING} {IN} TEMPLATE-FREE {PROTEIN} {LOOP} {STRUCTURE} MODELING: {AN} {OVERVIEW} [J] . Yaohang Li Computational and Structural Biotechnology Journal . 2013,第6期

机译：{CONFORMATIONAL} {SAMPLING} {IN}无模板的{PROTEIN} {LOOP} {STRUCTURE}建模：{AN} {概述}
4. {RECONSTRUCTION} {OF} {SAXS} {PROFILES} {FROM} {PROTEIN} {STRUCTURES} [J] . Daniel K. Putnam, Edward W. Lowe Jr., Jens Meiler Computational and Structural Biotechnology Journal . 2013,第11期

机译：{RECONSTRUCTION} {OF} {SAXS} {PROFILES} {FROM} {PROTEIN} {STRUCTURES}
5. Crystallization to obtain protein-ligand complexes for structure-aided drug design. [J] . Danley DE Acta crystallographica.Section D. Biological crystallography . 2006,第Pta6期

机译：结晶获得protein-ligand复合物structure-aided药物设计。
6. Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2 [D] . Yu, Qinhong. 2019

机译：Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2
7. Delicate conformational changes of a protein in the CRP family lead to dramatic functional changes via binding of an alternate secondary messenger molecule [O] . Chou, S.H. 2014

机译：Delicate conformational changes of a protein in the CRp family lead to dramatic functional changes via binding of an alternate secondary messenger molecule

Features of the secondary structure of a protein molecule from powder diffraction data.

摘要

著录项

相似文献

相关主题

期刊订阅