首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Enhancing MAD F(A) data for substructure determination.
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Enhancing MAD F(A) data for substructure determination.

机译:加强疯狂F为子结构(A)数据的决心。

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摘要

Heavy-atom substructure determination is a critical step in phasing an unknown macromolecular structure. Dual-space (Shake-and-Bake) recycling is a very effective procedure for locating the substructure (heavy) atoms using F(A) data estimated from multiple-wavelength anomalous diffraction. However, the estimated F(A) are susceptible to the accumulation of errors in the individual intensity measurements at several wavelengths and from inaccurate estimation of the anomalous atomic scattering corrections f' and f''. In this paper, a new statistical and computational procedure which merges multiple F(A) estimates into an averaged data set is used to further improve the quality of the estimated anomalous amplitudes. The results of 18 Se-atom substructure determinations provide convincing evidence in favor of using such a procedure to locate anomalous scatterers.
机译:是一个重原子子结构决心定相一个未知的至关重要的一步大分子的结构。(Shake-and-Bake)回收是一种非常有效的程序定位子结构(重型)原子使用F (A)数据估计多波长反常衍射。然而,估计F (A)是容易的在个人的积累误差在几个波长和强度的测量不准确的估计的异常原子散射修正f和f”。纸,一个新的统计和计算过程,合并多个F (A)的估计平均到一个数据集用于进一步提高估计的质量异常振幅。子结构决定提供令人信服的证据支持使用这样一个过程定位异常散射。

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