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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structural and functional analysis of Rv0554 from Mycobacterium tuberculosis: testing a putative role in menaquinone biosynthesis.
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Structural and functional analysis of Rv0554 from Mycobacterium tuberculosis: testing a putative role in menaquinone biosynthesis.

机译：Rv0554从结构和功能分析结核分枝杆菌:测试一个假定的甲基萘醌类生物合成的作用。

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摘要

Mycobacterium tuberculosis, the cause of tuberculosis, is a devastating human pathogen against which new drugs are urgently needed. Enzymes from the biosynthetic pathway for menaquinone are considered to be valid drug targets. The protein encoded by the open reading frame Rv0554 has been expressed, purified and subjected to structural and functional analysis to test for a putative role in menaquinone biosynthesis. The crystal structure of Rv0554 has been solved and refined in two different space groups at 2.35 and 1.9 A resolution. The protein is dimeric, with an alpha/beta-hydrolase monomer fold. In each monomer, a large cavity adjacent to the catalytic triad is enclosed by a helical lid. Dimerization is mediated by the lid regions. Small-molecule additives used in crystallization bind in the active site, but no binding of ligands related to menaquinone biosynthesis could be detected and functional assays failed to support possible roles in menaquinone biosynthesis.
机译：结核分枝杆菌的原因结核病是一个毁灭性的人类病原体迫切需要对新药。酶的生物合成途径甲基萘醌类被认为是有效的药物目标。帧Rv0554一直表达,纯化和进行结构和功能分析甲基萘醌类测试一个假定的角色生物合成。在两个不同的空间解决和完善组在2.35和1.9的决议。二聚的,一个α/ beta-hydrolase单体吗褶皱。催化三螺旋盖子是封闭的。二聚作用是受控制的地区。小分子添加剂用于结晶结合在活性位点,但没有绑定的甲基萘醌类相关配体的生物合成被发现和功能检测失败在甲基萘醌类支持可能的作用生物合成。

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