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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Real-time PCR: detection of oestrogen receptor-alpha and -beta isoforms and variants in breast cancer.
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Real-time PCR: detection of oestrogen receptor-alpha and -beta isoforms and variants in breast cancer.

机译：实时PCR：检测乳腺癌中的雌激素受体α和β异构体和变异体。

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摘要

BACKGROUND: Splice variants exist for both alpha and beta oestrogen receptors (ERs). Oestrogen function results from a balance between the wild-type ERs (wt) and their variants. PATIENTS AND METHODS: Forty formalin-fixed paraffin-embedded breast cancer samples were analysed by real-time PCR using ERalpha primer sets detecting wt and exon-deleted 3, 5, 6 and 7 variants. The ERbeta primer sets detected wt ERbeta1 and ERbeta2 and ERbeta5 variants. At the end of the PCR cycles, a dissociation curve was generated showing the peaks for each sample at specific melting temperatures (Tm); finding more than one peak indicated the presence of variants. RESULTS: Many samples expressed both wt ER isoforms and their variants. The Tm value served as a cut-off point for determination of wt versus variant ER expression. CONCLUSION: This method of detection of wt and variant ER could help in patient selection for anti-oestrogen therapy and in monitoring response to therapy.
机译：背景：α和β雌激素受体（ER）均存在剪接变体。雌激素功能来自野生型ER（wt）及其变异体之间的平衡。病人和方法：40份福尔马林固定石蜡包埋的乳腺癌样品通过实时PCR分析，使用ERalpha引物组检测wt和外显子缺失的3、5、6和7个变异。 ERbeta引物组检测到wt ERbeta1和ERbeta2和ERbeta5变体。在PCR循环结束时，产生了解离曲线，显示了每个样品在特定解链温度（Tm）下的峰。发现一个以上的峰表明存在变体。结果：许多样品均表达wt ER同工型及其变体。 Tm值用作确定wt与变体ER表达的临界点。结论：这种检测wt和ER变异体的方法可以帮助选择抗雌激素治疗的患者，并监测对治疗的反应。

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