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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >KRAS up-regulates the expression of miR-181a, miR-200c and miR-210 in a three-dimensional-specific manner in DLD-1 colorectal cancer cells.
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KRAS up-regulates the expression of miR-181a, miR-200c and miR-210 in a three-dimensional-specific manner in DLD-1 colorectal cancer cells.

机译：KRAS以三维特异性方式在DLD-1大肠癌细胞中上调miR-181a，miR-200c和miR-210的表达。

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摘要

We previously found that oncogenic KRAS induces increased expression of microRNAs (miRNAs), such as miR-200c and miR-221/222, in human colorectal cancer (CRC) HCT116 cells in a three-dimensional (3D)-specific manner, however, the regulation of miRNA expression through oncogenic KRAS in other types of CRC remains unclear.The differential expression of 94 cancer-related miRNAs was examined in DLD-1 and DKO-4 cells (DLD-1 cells with a disrupted oncogenic KRAS) in 3D cultures.Increased miR-15b, miR-16, miR-23a, miR-24, miR-103 and miR-222 expression was observed in 3D and in 2D cultures. Of note, increased miR-181a, miR-200c and miR-210 expression was only observed in 3D cultures. Furthermore, miR-181a and miR-210 were significantly overexpressed in DLD-1 cells in 3D culture compared with those in HCT116 cells, and were significantly overexpressed in human CRC specimens.Oncogenic KRAS regulates 3D-specific miRNAs that are possibly associated with CRC development in vivo.
机译：我们先前发现，致癌性KRAS以三维（3D）特异性方式诱导人结肠直肠癌（CRC）HCT116细胞中miRNA（miRNA）（例如miR-200c和miR-221 / 222）的表达增加，在其他类型的CRC中通过致癌性KRAS调节miRNA表达的调控仍不清楚。在3D培养物中的DLD-1和DKO-4细胞（致癌性KRAS被破坏的DLD-1细胞）中检测了94种与癌症相关的miRNA的差异表达。在3D和2D培养物中观察到miR-15b，miR-16，miR-23a，miR-24，miR-103和miR-222表达增加。值得注意的是，仅在3D培养物中观察到miR-181a，miR-200c和miR-210表达增加。此外，与HCT116细胞相比，miR-181a和miR-210在3D培养中的DLD-1细胞中显着过表达，并且在人CRC标本中显着过表达。致癌性KRAS调节可能与CRC发育有关的3D特异性miRNA。体内。

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