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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Mutations and aberrant transcriptions of Stk11 (Lkb1) gene in rat liver tumors.
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Mutations and aberrant transcriptions of Stk11 (Lkb1) gene in rat liver tumors.

机译：大鼠肝肿瘤中Stk11（Lkb1）基因的突变和异常转录。

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摘要

To clarify the involvement of the Stk11/Lkb1 gene in the development of hepatocellular carcinomas (HCCs), its alteration in rat HCCs induced by exogenous and endogenous liver carcinogenesis models was investigated. MATERIALS AND METHODS: Fifteen HCCs induced by N-nitrosodiethylamine (DEN) and 12 HCCs induced by a choline-deficient L-amino acid-defined (CDAA) diet were obtained. To assess mutations and aberrant transcriptions of the Stk11 gene, polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and reverse transcription (RT)-PCR analyses were performed, respectively. RESULTS: A mutation was detected in only 1 out of 15 HCCs by DEN, but no mutations in 12 HCCs by the CDAA diet. Aberrant transcripts were found in 4 out of 15 HCCs by DEN and in 3 out of 12 HCCs by the CDAA diet. CONCLUSION: These results suggest that alterations of the Stk11 gene may play a limited role in both exogenous and endogenous rat liver carcinogenesis.
机译：为了阐明Stk11 / Lkb1基因参与肝细胞癌（HCCs）的发展，研究了外源性和内源性肝癌模型在大鼠HCC中的改变。材料与方法：获得了15种由N-亚硝基二乙胺（DEN）诱导的HCC和12种由胆碱缺乏L-氨基酸定义（CDAA）饮食诱导的HCC。为了评估Stk11基因的突变和异常转录，分别进行了聚合酶链反应（PCR）-单链构象多态性（SSCP）和逆转录（RT）-PCR分析。结果：DEN仅在15个HCC中检测到1个突变，而CDAA饮食在12个HCC中未检测到突变。 DEN发现15个HCC中有4个异常转录本，而CDAA饮食中12个HCC中有3个发现异常转录本。结论：这些结果表明Stk11基因的改变可能在外源性和内源性大鼠肝癌发生中起有限的作用。

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