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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Revitalization of human bone after extracorporeal high hydrostatic pressure treatment.
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Revitalization of human bone after extracorporeal high hydrostatic pressure treatment.

机译：体外高静水压治疗后的人骨活化。

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BACKGROUND: Bone defects due to malignant tumor often lead to repeated surgery or amputation. Thus, a major objective in orthopedic surgery is the extracorporeal devitalization of tumor-bearing bone segments following reimplantation. Extracorporeal irradiation or autoclaving are possible methods even though they may cause severe loss of biomechanical and biological properties. Yet previous studies have shown that high hydrostatic pressure (HHP) allows for complete devitalization of tumor-afflicted bone segments, while the biomechanical and biological properties of bone tissue remained unchanged. The subject of the present study is revitalization of human bone segments after HHP treatment to acquire knowledge about the ingrowth and regeneration of osteoblast-like cells after such treatment. MATERIALS AND METHODS: Bone pieces of 5 mm(3) were obtained from cancellous bone, taken from human femoral heads of 6 patients undergoing surgery for total hip arthroplasty, and exposed to hydrostatic pressure levels of 0, 300, and 600 MPa for 10 min at 37 degrees C. Following the HHP treatment, bone segments were coated with primary human bone cells (10,000 cells/segment), cultured for 42 days and cell viability and proliferation quantified at different time points. RESULTS: An adhesion rate of 73.8% on day 1 and an increase in proliferation between day 14 and 42 were determined. Pretreatment of bone segments with 300 and 600 MPa did not affect cell adhesion or proliferation. Histology showed intact cells and new bone formation on the bone specimens; elevated expression of alkaline phosphatase, osteocalcin, and collagen type I was seen by immunohistochemistry. CONCLUSION: The present study demonstrates, for the first time, the successful revitalization of HHP-treated bone segments. Concerning proliferation and osteogenic differentiation, the findings are a promising demonstration of sufficient osseointegration. Along with previous results, we anticipate that a pressure of a maximum 350 MPa does induce devitalization of malignant bone tumor segments, while HHP treatment of bone matrix up to 600 MPa does not affect osteoconductivity and osteoinductivity.
机译：背景：由于恶性肿瘤引起的骨缺损通常会导致重复手术或截肢。因此，整形外科的主要目的是在移植后对荷瘤骨段进行体外灭活。体外照射或高压灭菌是可能的方法，即使它们可能会严重破坏生物力学和生物学特性。然而，先前的研究表明，高静水压（HHP）可以使受肿瘤困扰的骨段完全失去活力，而骨组织的生物力学和生物学特性却保持不变。本研究的主题是HHP处理后的人骨段的活化，以获取有关这种处理后成骨样细胞向内生长和再生的知识。材料与方法：5毫米（3颗）骨取自松质骨，取自6例行全髋关节置换术的患者的股骨头，并在0、300和600 MPa的静水压力下暴露10分钟在HHP处理之后，在37摄氏度下进行。将骨段涂上人类原代骨细胞（10,000个细胞/段），培养42天，并在不同时间点对细胞活力和增殖进行定​​量。结果：在第1天，粘附率达到73.8％，在第14天和第42天之间，增殖增加。用300和600 MPa的骨段进行预处理不会影响细胞粘附或增殖。组织学显示完整的细胞和新的骨形成在骨标本上。免疫组织化学观察到碱性磷酸酶，骨钙素和Ⅰ型胶原蛋白表达升高。结论：本研究首次证明了HHP治疗的骨段的成功再生。关于增殖和成骨分化，该发现是充分骨整合的有希望的证明。与以前的结果一起，我们预计最大350 MPa的压力确实会诱发恶性骨肿瘤段的失活，而对高达600 MPa的骨基质进行HHP处理不会影响骨传导性和骨诱导性。

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