Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression.

Kuramitsu Y; Hayashi E; Okada F; Zhang X; Ueyama Y; Nakamura K

首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression.

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Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression.

机译：使用固定的pH梯度条和FlamingoTM荧光凝胶染色剂进行的二维凝胶电泳鉴定了可能与恶性肿瘤进展相关的非核蛋白。

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The understanding of tumour progression is one of the most important strategies to conquer tumour. QR-32 is a regressive murine fibrosarcoma cell line, and QRsP-11 is a progressive malignant tumour cell clone derived from QR-32. Ina recently published study a differential display analysis for the cytoplasmic proteins was shown by using two-dimensional gel electrophoresis (2-DE) making full use of isoelectric focusing capillary gels and Coomassie brilliant blue R-250 staining. Furthermore, a differential display analysis of the nuclear proteome for QR-32 and QRsP-11 was performed. The present study shows a non-nuclear proteomic differential display analysis, using 2-DE making full use of immobilized pH gradient strips and Flamingo fluorescent gel stain, between QR-32 and QRsP-11 to identify particular proteins which may be involved in malignant progression. In QRsP-11 25 proteins were up-regulated, including hypoxia up-regulated protein 1, and 6 were down-regulated compared with QR-32. These results suggest that the identified non-nuclear proteins showing different expression between QR-32 and QRsP-11 possibly related to malignant tumour progression.

机译：了解肿瘤的进展是征服肿瘤的最重要策略之一。 QR-32是一种鼠类纤维肉瘤的退化细胞系，QRsP-11是一种源自QR-32的进行性恶性肿瘤细胞克隆。在最近发表的一项研究中，利用二维凝胶电泳（2-DE）充分利用了等电聚焦毛细管凝胶和考马斯亮蓝R-250染色，显示了胞质蛋白的差异显示分析。此外，对QR-32和QRsP-11的核蛋白质组进行了差异显示分析。本研究显示了无核蛋白质组差异显示分析，利用2-DE充分利用固定化的pH梯度条和Flamingo荧光凝胶染色剂在QR-32和QRsP-11之间鉴定可能与恶性进展有关的特定蛋白质。与QR-32相比，QRsP-11中的25种蛋白质上调，包括缺氧上调的蛋白质1和6种下调。这些结果表明，鉴定出的非核蛋白在QR-32和QRsP-11之间表现出不同的表达，可能与恶性肿瘤的进展有关。

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《Anticancer Research: International Journal of Cancer Research and Treatment》 |2011年第4期|共5页
作者
Kuramitsu Y; Hayashi E; Okada F; Zhang X; Ueyama Y; Nakamura K;
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正文语种 eng
中图分类肿瘤学;
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1. Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression. [J] . Kuramitsu Y, Hayashi E, Okada F, Anticancer Research: International Journal of Cancer Research and Treatment . 2011,第4期

机译：使用固定的pH梯度条和FlamingoTM荧光凝胶染色剂进行的二维凝胶电泳鉴定了可能与恶性肿瘤进展相关的非核蛋白。
2. ELECTROPHORETIC ANALYSIS OF BILIARY PROTEINS - APPLICATION OF HIGH RESOLUTION TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENT IN THE FIRST DIMENSION [J] . He C., Jungst D. Electrophoresis: The Official Journal of the International Electrophoresis Society . 1996,第3期

机译：胆汁蛋白质的电泳分析-固定化pH梯度的高分辨率二维聚丙烯酰胺凝胶电泳在第一维中的应用
3. A comparative evaluation of rehydration and cup-loading sample application for modified two-dimensional gel electrophoresis of human serum proteins using immobilized pH gradient [J] . Ali Mostafaie, Kheirollah Yari, Sara Kiani African Journal of Biotechnology . 2011,第55期

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4. Separation and Detection of Urinary Proteins by Two-dimensional Non-gel Sieving Electrophoresis on the Microfluidic Chip [C] . Yanan Ni, Jun Gan, Yi Xu, The 14th Annual conference of the Chinese Society of Micro-Nano technology; The 3rd International conference of the Chinese Society of Micro-Nano technology; The 6th International workshop on innovation and commercialization of Micro amp; Nanotechnology . 2012

机译：微流控芯片上二维非凝胶筛分电泳分离和检测尿蛋白
5. Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis. [D] . Anduri, Sridevi. 2009

机译：使用一维和二维凝胶电泳确定正常和两性男性小口黑鲈的差异蛋白表达谱。
6. Identification of Proteins Related to Nickel Homeostasis in Helicobater pylori by Immobilized Metal Affinity Chromatography and Two-Dimensional Gel Electrophoresis [O] . Xuesong Sun, Ruiguang Ge, Jen-Fu Chiu, 2008

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7. Fluorescent two-dimensional difference gel electrophoresis and mass spectrometry identify age-related protein expression differences for the primary visual cortex of kitten and adult cat [O] . Gert Van den Bergh, Stefan Clerens, Lieselotte Cnops, 2003

机译：荧光二维差异凝胶电泳和质谱鉴定小猫和成年猫的原发性视觉皮层的年龄相关蛋白表达差异
8. Two-Dimensional Micro-Separation Technique for Proteins and Peptides, Combining Isoelectric Focusing and Gel Gradient Electrophoresis. [R] . Ruechel, R. 1976

机译：蛋白质和多肽的二维微分离技术，结合等电聚焦和凝胶梯度电泳。

Two-dimensional gel electrophoresis using immobilized pH gradient strips and FlamingoTM fluorescent gel stain identified non-nuclear proteins possibly related to malignant tumour progression.

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