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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >From electron microscopy to X-ray crystallography: molecular-replacement case studies.
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From electron microscopy to X-ray crystallography: molecular-replacement case studies.

机译：从电镜x射线晶体学:molecular-replacement案例研究。

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摘要

Multi-component molecular complexes are increasingly being tackled by structural biology, bringing X-ray crystallography into the purview of electron-microscopy (EM) studies. X-ray crystallography can utilize a low-resolution EM map for structure determination followed by phase extension to high resolution. Test studies have been conducted on five crystal structures of large molecular assemblies, in which EM maps are used as models for structure solution by molecular replacement (MR) using various standard MR packages such as AMoRe, MOLREP and Phaser. The results demonstrate that EM maps are viable models for molecular replacement. Possible difficulties in data analysis, such as the effects of the EM magnification error, and the effect of MR positional/rotational errors on phase extension are discussed.
机译：多组分的分子复合物逐渐被解决通过结构生物学,将x射线结晶学的范围电子显微镜(EM)的研究。晶体学可以利用低分辨率地图结构确定随后的阶段扩展高分辨率。进行5晶体结构大的分子组装,EM地图用作结构模型的解决方案分子置换(MR)使用不同的标准包先生如爱慕,MOLREP和移相器。结果表明,地图是可行的模型分子置换。数据分析的困难,如EM放大误差的影响,位置/旋转错误先生的影响阶段扩展进行了讨论。

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