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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The use of biophysical methods increases success in obtaining liganded crystal structures.
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The use of biophysical methods increases success in obtaining liganded crystal structures.

机译：生物物理方法的使用增加成功在获得配体晶体结构。

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摘要

In attempts to determine the crystal structure of small molecule-protein complexes, a common frustration is the absence of ligand binding once the protein structure has been solved. While the first structure, even with no ligand bound (apo), can be a cause for celebration, the solution of dozens of apo structures can give an unwanted sense of deja vu. Much time and material is wasted on unsuccessful experiments, which can have a serious impact on productivity and morale. There are many reasons for the lack of observed binding in crystals and this paper highlights some of these. Biophysical methods may be used to confirm and optimize solution conditions to increase the success rate of crystallizing protein-ligand complexes. As there are an overwhelming number of biophysical methods available, some of the factors that need to be considered when choosing the most appropriate technique for a given system are discussed. Finally, a few illustrative examples where biophysical methods have proven helpful in real systems are given.
机译：在试图确定的晶体结构小分子蛋白质复合体,一个共同的挫折没有配体绑定一次蛋白质结构已经解决。第一个结构,即使没有配体(apo),可能是一个庆祝的理由,解决吗数十名apo结构可以给一个不受欢迎的似曾相识的感觉。浪费在不成功的实验,可以有一个严重的影响生产力和士气。有许多原因缺乏观察绑定在晶体和本文亮点其中的一些。确认条件和优化解决方案增加结晶的成功率protein-ligand复合物。绝大多数的生物物理方法可用,需要的一些因素考虑选择最合适的对于一个给定的系统技术进行了讨论。最后,一些说明性的例子生物物理的方法已被证明有助于真实的系统。

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