...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Methylation-mediated silencing of TMS1 in pancreatic cancer and its potential contribution to chemosensitivity.
【24h】

Methylation-mediated silencing of TMS1 in pancreatic cancer and its potential contribution to chemosensitivity.

机译：甲基化介导的TMS1在胰腺癌中的沉默及其对化学敏感性的潜在贡献。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Resistance to chemotherapeutic agents, resulting in part from epigenetic silencing of pro-apoptotic genes, is one of the causes of treatment failure of pancreatic cancer. We examined whether epigenetic silencing of target of methylation induced silencing 1 (TMS1) contributes to resistance to chemotherapy in pancreatic cancer. MATERIALS AND METHODS: Methylation analysis was performed by methylation-specific PCR (MS-PCR) and gene expression was analyzed by quantitative reverse transcriptase PCR (qRT-PCR). MIA PaCa-2 cells were transfected with pCMV6-XL5/TMS1 plasmid and the effect of TMS1 expression on sensitivity to gemcitabine and docetaxel was determined. Cell viability was measured using Cell Titer Blue assay. RESULTS: TMS1 expression was repressed in MIA PaCa-2 cells by DNA methylation. Up-regulation of TMS1 by recombinant gene expression in MIA PaCa-2 cells or by pre-treatment of these cells with 5-azacytidine resulted in enhanced sensitivity to gemcitabine and docetaxel. CONCLUSION: Our results suggest that TMS1 is a potential therapeutic target in pancreatic cancer.
机译：背景：对化学治疗药物的抗性，部分是由于促凋亡基因的表观遗传沉默所致，是胰腺癌治疗失败的原因之一。我们检查了甲基化诱导的沉默1（TMS1）的目标的表观遗传沉默是否有助于对胰腺癌的化疗耐药。材料与方法：通过甲基化特异性PCR（MS-PCR）进行甲基化分析，并通过定量逆转录酶PCR（qRT-PCR）分析基因表达。用pCMV6-XL5 / TMS1质粒转染MIA PaCa-2细胞，并测定TMS1表达对吉西他滨和多西他赛敏感性的影响。使用细胞滴度蓝测定法测量细胞活力。结果：通过DNA甲基化抑制了MIA PaCa-2细胞中TMS1的表达。通过在MIA PaCa-2细胞中重组基因表达或通过用5-氮杂胞苷预处理这些细胞，可以上调TMS1对吉西他滨和多西他赛的敏感性。结论：我们的结果表明TMS1是胰腺癌的潜在治疗靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号