Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)

Ozbek S; Muller JF; Figgemeier EStetefeld J

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Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)

机译：有利的水晶联系的中介cocoamidopropylbetaine(变动)

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Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 angstrom resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 angstrom.

机译：晶体的优秀质量的先决条件高分辨率的x射线数据。结晶细化协议条件通常很难获得正确的组合,例如,蛋白质浓度、滴大小、温度和添加剂。水晶接触最有利的方式执行结晶的设置两性离子表面活性剂cocoamidoproylbetaine(变动)。表面活性剂、高衍射晶体获得的。线圈(RHCC)在复杂的变动为1.4埃提出了解决。否则使用相同的结晶条件和相同的x射线源造成了改善决议从2.9到1.4埃。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2005年第4期|477-480|共4页
作者
Ozbek S; Muller JF; Figgemeier EStetefeld J;
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作者单位

Univ Basel, Biozentrum, Dept Biophys Chem, CH-4056 Basel, Switzerland;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Mediation; Coiled-Coil Domain; zwitterionic surfactantcrystalsAngstromCrystallization;

机译：中介;卷曲螺旋域;两性离子surfactantcrystalsAngstromCrystallization;

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Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)

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