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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Getting the most out of X-ray home sources
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Getting the most out of X-ray home sources

机译：得到的x射线源

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The structures of a 14 kDa phospholipase, an 18 kDa proteinase inhibitor and a novel glycoside hydrolase with molecular weight 60 kDa were solved using the SAD technique and the effects of the amount of anomalous signal, completeness and redundancy of data on heavy-atom substructure determination, phasing and model building were analyzed. All diffraction data sets were collected on a Cu-anode X-ray home source. The structure of the phospholipase was obtained using the anomalous scattering contribution from its 16 S atoms. Three-dimensional models for the other two macromolecules were obtained using the anomalous contribution of I atoms rapidly incorporated into the crystal through the quick cryo-soaking method of derivatization. These results were used to discuss the application of sulfur- and iodine-SAD approaches in combination with X-ray home sources for high-throughput protein crystal structure solution. The estimates of the anomalous signal from S atoms in the gene products of four genomes are given and the prospects for increasing the anomalous contribution using longer wavelengths (e.g. from a chromium home source) and quick cryo-soaking derivatization are discussed. The possibility of rapidly preparing tangible home-source isomorphous derivatives suggests that this approach might become a valuable tool in the future of post-genomic projects.
机译：14的结构kDa磷脂酶,18岁kDa蛋白酶抑制剂和一种新型糖苷水解酶分子量60 kDa解决了用悲伤的技术和所带来的影响的异常信号,完整性和冗余的数据重原子子结构决心,逐步和模型构建分析。收集在一个Cu-anode x射线源。磷脂酶的结构得到了使用从16反常散射的贡献S原子。两种大分子得到使用迅速异常的贡献我的原子通过快速融入水晶cryo-soaking衍生化的方法。结果被用来讨论的应用硫和iodine-SAD方法相结合与x射线来源高通量蛋白质晶体结构的解决方案。异常信号S原子的基因产品的四个基因组和增加异常的前景使用长波长(例如从贡献一个铬源)和快速cryo-soaking回家衍生化作用进行了讨论。迅速准备有形home-source同晶型衍生品表明这一点方法可能成为一个有价值的工具未来的之一。

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