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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of a putative 2'-5' RNA ligase from Pyrococcus horikoshii.
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Structure of a putative 2'-5' RNA ligase from Pyrococcus horikoshii.

机译：假定的2 ' 5 ' RNA连接酶的结构Pyrococcus horikoshii。

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摘要

Cyclic phosphodiesterase and 2'-5' RNA ligase are members of a superfamily of proteins which share structural similarities even though their homology may be very low. A putative 2'-5' RNA ligase from Pyrococcus horikoshii has been crystallized and its X-ray crystallographic structure determined to 2.4 A. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.07, b = 45.47, c = 93.17 A and one protein monomer in the asymmetric unit. The molecular-replacement probe was a 2'-5' RNA ligase from Thermus thermophilus which shares 30% sequence identity. The P. horikoshii RNA ligase has some structural features that have more in common with a cyclic phosphodiesterase from Arabidopsis thaliana with which it has no significant homology, yet an examination of the electrostatic surface potential clearly defines its relationship to the T. thermophilus RNA ligase. However, the size of the active-site cleft is smaller and less positively charged than that of the T. thermophilus homologue, suggesting that the actual substrate may be smaller than that previously postulated for the latter.
机译：环磷酸二酯酶和2 ' 5 ' RNA连接酶的蛋白质超家族的成员分享尽管他们的结构相似之处同源性可能很低。连接酶从海床horikoshii结晶及其x射线晶体2.4结构的决心。斜方晶系的空间组的结晶P2(1) 2(1) 2(1),晶胞参数=44.07, b = 45.47, c = 93.17,一个蛋白质单体的不对称单元。molecular-replacement探针2 ' 5 ' RNA连接酶从栖热菌属酸奶有助于股票的30%序列的身份。有一些结构特点,有更多的吗常见的环磷酸二酯酶它没有的拟南芥显著的同源性,但考试的表面静电势清晰定义t .酸奶RNA的关系连接酶。带正电的间隙较小,小于t .酸奶的同系物,暗示实际的基质可能小于之前假设后者。

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