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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Cadherin-6 gene expression in conventional renal cell carcinoma: a useful marker to detect circulating tumor cells.
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Cadherin-6 gene expression in conventional renal cell carcinoma: a useful marker to detect circulating tumor cells.

机译：常规肾细胞癌中Cadherin-6基因表达：检测循环肿瘤细胞的有用标志物。

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摘要

BACKGROUND: Dissemination of cancer cells into the circulation is an essential step in the development of a metastasis. Detection of circulating cancer cells may improve the monitoring methods for cancer patients. However, the detection of circulating renal cancer cells is mainly hampered by the lack of markers available for renal cell carcinoma (RCC). In this study, we evaluated cadherin-6 mRNA as a new molecular marker for the detection of circulating renal cancer cells. MATERIALS AND METHODS: Forty-six blood samples of conventional RCCs were included. A standard protocol of RT-PCR, assisted by computer densitometric analysis to establish a cut-off, was performed to examine cadherin-6 mRNA expression by using specific primers. A renal cancer cell line, SKRC-59 and forty tumor biopsies from conventional RCCs were used as positive controls. Twenty-five blood samples from non-RCC patients were also analyzed. RESULTS: Cadherin-6 mRNA could be detected in 38140 (95%) conventional RCC specimens. Cadherin-6mRNA was positive in 21/46 (45.7%) blood samples of RCC patients, while no positivity was found in non-RCC blood samples. Among the localized RCCs, 14/35 (40.0%) blood samples were positive while 7/11 (63.6%) were positive among the blood samples from metastatic RCCs. CONCLUSION: Our data indicate that cadherin-6 gene is frequently expressed in conventional RCCs. Cadherin-6 is a useful molecular marker to detect the circulating cancer cells disseminated from conventional RCC.
机译：背景：将癌细胞扩散到循环中是转移发生过程中必不可少的步骤。循环癌细胞的检测可以改善对癌症患者的监测方法。但是，主要由于缺乏可用于肾细胞癌（RCC）的标记而阻碍了循环肾癌细胞的检测。在这项研究中，我们评估了钙黏着蛋白6 mRNA作为检测循环肾癌细胞的新分子标记。材料与方法：包括常规RCC的46份血液样本。通过使用特定引物，进行了RT-PCR的标准方案，并借助计算机光度法分析建立了临界值，以检测钙黏着蛋白6 mRNA的表达。肾癌细胞系，SKRC-59和来自常规RCC的40份肿瘤活检被用作阳性对照。还分析了来自非RCC患者的25份血液样本。结果：在38140例（95％）常规RCC标本中可以检测到Cadherin-6 mRNA。在RCC患者的21/46（45.7％）血液样本中，Cadherin-6mRNA呈阳性，而在非RCC血液样本中未发现阳性。在局部RCC中，转移RCC的血液样品中14/35（40.0％）为阳性，而7/11（63.6％）为阳性。结论：我们的数据表明，钙黏着蛋白6基因在常规RCC中频繁表达。 Cadherin-6是检测从常规RCC传播的循环癌细胞的有用分子标记。

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