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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Response of PC-3 prostate cancer cells to combination therapy using irradiation with glucocorticoids or doxorubicin.
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Response of PC-3 prostate cancer cells to combination therapy using irradiation with glucocorticoids or doxorubicin.

机译：PC-3前列腺癌细胞对使用糖皮质激素或阿霉素照射的联合治疗的反应。

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OBJECTIVES: We evaluated the effects of irradiation, doxorubicin and dexamethasone on human PC-3 prostate cancer cells, investigating whether dexamethasone and doxorubicin can alter the irradiation cytotoxicity of PC-3 cells. METHODS: We used the human PC-3 prostate cancer cells, analyzing cell growth with trypan blue exclusion, indices of the cell cycle with flow cytometry and apoptosis with flow cytometry and analysis of DNA fragmentation on simple agarose gel. RESULTS: Doxorubicin (100 nM) arrested cell cycle at the G2/M phase, decreased cell growth and produced apoptosis of PC-3 cells in a time-dependent manner. Dexamethasone (100 nM) increased the distribution of PC-3 cells at G0/G1 phase in the cell cycle, exerting an inhibitory effect on the proliferation of PC-3 cells after 48 and 72 hr, but it did not produce apoptosis. Irradiation (4 Gy) initially arrested cells at the G2/M phase in the cell cycle (24 hr) which was gradually overcome and the PC-3 cells were shifted into G0/G1 phase or apoptosis after 48 and 72 hr. Irradiation decreased the PC-3 cell growth by 40-50% after 48 and 72 hr, respectively. Treatment with doxorubicin (100 nM) for 24, 48, and 72 hr after irradiation potentiated irradiation cytotoxicity of PC-3 cells. Dexamethasone treatment 24 hr before and 24, 48 and 72 hr after irradiation increased the number of surviving PC-3 cells and partially neutralized the irradiation effects on cell cycle. CONCLUSION: Doxorubicin potentiated while dexamethasone partially reversed the irradiation cytotoxicity of PC-3 cells. These data may be of clinical importance for the treatment of hormone refractory prostate cancer.
机译：目的：我们评估了辐射，阿霉素和地塞米松对人PC-3前列腺癌细胞的影响，研究了地塞米松和阿霉素是否可以改变PC-3细胞的辐射细胞毒性。方法：我们使用人PC-3前列腺癌细胞，用锥虫蓝排除法分析细胞生长，用流式细胞术分析细胞周期指标，用流式细胞术分析细胞凋亡，并在简单的琼脂糖凝胶上分析DNA片段。结果：阿霉素（100 nM）在G2 / M期停滞了细胞周期，减少了细胞的生长，并以时间依赖性的方式产生了PC-3细胞的凋亡。地塞米松（100 nM）在细胞周期中增加了PC-3细胞在G0 / G1期的分布，在48和72小时后对PC-3细胞的增殖具有抑制作用，但未产生凋亡。辐射（4 Gy）最初将细胞停滞在细胞周期（24小时）的G2 / M期，并逐渐被克服，并且PC-3细胞在48和72小时后转变为G0 / G1期或凋亡。照射分别在48和72小时后使PC-3细胞的生长减少了40-50％。辐射后用阿霉素（100 nM）处理24、48和72小时，可增强PC-3细胞的辐射细胞毒性。地塞米松治疗在辐射前24小时，辐射后24、48和72小时增加了存活的PC-3细胞的数量，并部分中和了辐射对细胞周期的影响。结论：阿霉素增强而地塞米松部分逆转了PC-3细胞的辐射细胞毒性。这些数据对于激素难治性前列腺癌的治疗可能具有临床重要性。

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