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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Sugar-mediated lattice contacts in crystals of a plant glycoprotein.
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Sugar-mediated lattice contacts in crystals of a plant glycoprotein.

机译：Sugar-mediated晶格联系人的晶体植物糖蛋白。

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摘要

Pokeweed antiviral protein, PAP-S(aci), isolated from seeds of the Chinese pokeweed plant, Phytolacca acinosa, belongs to the family of type-1 ribosome-inactivating proteins (RIPs). Type-1 RIPs are approximately 30-kDa N-glycosidases that inactivate eukaryotic and prokaryotic ribosomes via a site-specific depurination of ribosomal RNA (rRNA). Here we describe the preliminary X-ray structure determination at 1.7 A resolution of one PAP isoenzyme from seeds, PAP-S1(aci), after crystallisation from a heterogeneous mixture of two isoenzymes. PAP-S1(aci) possesses a rare type of glycosylation, specifically, N-linked N-acetyl-D-glucosamine monosaccharide (GlcNAc) substitutions at canonical Asn-Xaa-Ser/Thr sequons. One GlcNAc residue was found to play a critical role in crystal lattice formation, forming a packing interface across a crystallographic two-fold with the identical sequon of an adjacent monomer. This observation suggests that deglycosylation protocols for the crystallisation of glycoproteins should be designed to allow for exploitation of the crystal packing potential of the innermost core sugar residue (N-linked GlcNAc or O-linked GalNAc).
机译：商陆抗病毒蛋白,PAP-S (aci)、孤立中国商陆种子的植物,商acinosa,属于家族1型ribosome-inactivating蛋白质(撕裂)。1型约30-kDa撕裂N-glycosidases真核和灭活原核生物的核糖体通过特定站点脱嘌呤的核糖体RNA (rRNA)。描述了初步的x射线结构确定在1.7解决PAP同工酶从种子、PAP-S1 (aci)结晶的不均匀混合物两个同功酶。糖基化,具体来说,N-linkedN-acetyl-D-glucosamine单糖(GlcNAc)在正则替换Asn-Xaa-Ser /刺sequons。在晶格的形成至关重要的作用,形成一个在包装接口晶体的双重的相同sequon相邻的单体。表明deglycosylation协议的结晶的糖蛋白旨在允许剥削的晶体包装的潜力最里面的核心糖残渣(N-linked GlcNAc或O-linked GalNAc)。

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