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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >From conventional crystallization to better crystals from space: a review on pilot crystallogenesis studies with aspartyl-tRNA synthetases.
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From conventional crystallization to better crystals from space: a review on pilot crystallogenesis studies with aspartyl-tRNA synthetases.

机译：与传统的结晶更好从太空中晶体:回顾飞行员crystallogenesis研究aspartyl-tRNAsynthetases。

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摘要

Aspartyl-tRNA synthetases were the model proteins in pilot crystallogenesis experiments. They are homodimeric enzymes of Mr approximately 125 kDa that possess as substrates a transfer RNA, ATP and aspartate. They have been isolated from different sources and were crystallized either as free proteins or in association with their ligands. This review discusses their crystallisability with emphasis to crystal quality and structure determination. Crystallization in low diffusivity gelled media or in microgravity environments is highlighted. It has contributed to prepare high-resolution diffracting crystals with better internal order as reflected by their mosaicity. With AspRS from Thermus thermophilus, the better crystalline quality of the space-grown crystals within APCF is correlated with higher quality of the derived electron density maps. Usefulness for structural biology of targeted methods aimed to improve the intrinsic physical quality of protein crystals is highlighted.
机译：Aspartyl-tRNA蛋白质合成酶是模型在飞行员crystallogenesis实验。homodimeric先生大约125 kDa的酶,拥有作为转移核糖核酸底物,ATP和天冬氨酸。不同来源,要么是结晶免费的蛋白质或与他们配体。crystallisability与强调晶体质量和结构的决心。结晶在低扩散系数稠化媒体或在微重力环境中突出显示。它导致了高分辨率做准备衍射晶体与更好的内部秩序mosaicity反映了。栖热菌属酸奶,结晶越好在APCF空间长大晶体的质量与更高质量的派生的吗电子密度图。旨在提高生物学的、有针对性的方法内在物理蛋白质晶体质量突出显示。

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