Screening crystallisation conditions using fluorescence correlation spectroscopy.

Schmauder R; Schmidt T; Abrahams JPKuil ME

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Screening crystallisation conditions using fluorescence correlation spectroscopy.

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Screening crystallisation conditions using fluorescence correlation spectroscopy.

机译：筛选结晶条件下使用荧光相关光谱。

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We investigate the potential of fluorescence correlation spectroscopy (FCS) in screening for crystallisation conditions. Solutions that nucleate protein crystals must have different interactions than solutions that do not give rise to crystals. Due to these different interactions the average mean squared displacement of the individual proteins changes. By monitoring protein self-diffusion, we can distinguish crystallizing from non-crystallizing solutions. The method introduced can be applied at extremely low concentrations in femtoliter volumes as an early diagnostic for molecular association. Based on our preliminary findings FCS has the potential to become a routine screening method for crystallography.

机译：我们研究荧光的潜力相关光谱(FCS)筛查结晶条件。有核的蛋白质晶体一定不同比解决方案不产生相互作用晶体。的平均均方位移个别蛋白质的变化。蛋白质自扩散,我们可以区分结晶从non-crystallizing解决方案。介绍的方法可以应用在极作为一个低浓度在飞升卷早期诊断分子协会。在我们的初步研究结果FCS的潜力成为一个常规筛查方法晶体学。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第1期|1536-1541|共6页
作者
Schmauder R; Schmidt T; Abrahams JPKuil ME;
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作者单位

Biophysics, Leiden University, PO BOX 9504, 2300 RA Leiden, The Netherlands.;

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正文语种英语
中图分类化学;
关键词
Crystallography; Spectrometry; low concentrationsMolecular associationvitrificationfluorescence correlation spectroscopyFluorescenceCrystallization;

机译：结晶学;光谱;低concentrationsMolecularassociationvitrificationfluorescence相关性spectroscopyFluorescenceCrystallization;

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Screening crystallisation conditions using fluorescence correlation spectroscopy.

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