Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

Nummelin H; El Tahir Y; Ollikka PSkurnik MGoldman A

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

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Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

机译：的表达、纯化和结晶collagen-binding片段的鼠疫adhesin

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A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype O:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A resolution on a synchrotron-radiation source.

机译：一个collagen-binding片段的重组形式鼠疫的enterocolitica O血清型:3 adhesinYadA氨基端polyhistidine亲和力标签已经在大肠杆菌生产,使用sitting-drop提纯和结晶vapour-diffusion技术。三角形空间群R3,晶胞参数a = b = 67.05, c = 221.95,衍射分辨率在1.55电脑同步源。

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《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|1042-1044|共3页
作者
Nummelin H; El Tahir Y; Ollikka PSkurnik MGoldman A;
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作者单位

Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Viikinkaari 1, PO Box 65, FIN-00014 University of Helsinki, Finland.;

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正文语种英语
中图分类化学;
关键词
Yersinia; Synchrotron radiation sources; adhesinsBacterial ProteinsCrystallization;

机译：Yersinia;Synchrotron radiation来源;adhesinsBacterial ProteinsCrystallization;

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Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

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