Reliable quality-control methods for protein crystal structures.

Badger J; Hendle J

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Reliable quality-control methods for protein crystal structures.

机译：可靠的蛋白质质量控制方法晶体结构。

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The emergence of structure-determination initiatives that employ high-throughput protein crystallography emphasizes the need to establish quality-control methods for screening the resulting models prior to deposition with the public data banks. An in-house database of 26 new protein structures, associated diffraction data and high-quality experimentally determined electron-density maps have been used to develop (i) a set of minimal global quality criteria that a structure must meet before the refinement may be considered completed and (ii) a reliable set of indicators for detecting local errors in protein structures. These criteria have been applied to detecting local errors to a set of structures recently deposited in the Protein Data Bank and it is estimated that about 3% of amino acids are incorrectly modeled.

机译：结构测定的出现计划采用高通量的蛋白质晶体学强调需要建立质量控制方法筛查沉积与之前产生的模型公共数据银行。蛋白质结构、关联衍射数据和高质量的实验确定电子密度图已经被用于开发(我)一组最小的全球质量标准前一个结构必须满足细化被认为是和(2)一套可靠的完成指标检测本地错误蛋白质的结构。应用于检测本地错误的一组最近存入蛋白质结构数据银行,据估计,大约3%的氨基酸酸是不正确地建模。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2002年第2期|284-291|共8页
作者
Badger J; Hendle J;
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作者单位

Structural GenomiX, 10505 Roselle Street, San Diego, California 92121, USA. john_badger@stromix.com;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
computer software; Database; Total Quality ManagementProtein Data BankProteinControl Methodscomputer programsQuality managementLocal errorsQuality Control;

机译：计算机软件、数据库、总质量ManagementProtein数据BankProteinControlMethodscomputer programsQuality managementLocalerrorsQuality控制;

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Reliable quality-control methods for protein crystal structures.

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