...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Oxidative stress reduces Na+/H+ exchange (NHE) activity in a biliary epithelial cancer cell line (Mz-Cha-1).
【24h】

Oxidative stress reduces Na+/H+ exchange (NHE) activity in a biliary epithelial cancer cell line (Mz-Cha-1).

机译：氧化应激会降低胆道上皮癌细胞系（Mz-Cha-1）中的Na + / H +交换（NHE）活性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In cholangiocarcinogenesis, chronic inflammation and oxidative stress play a key role. The Na(+)/H(+) exchanger (NHE) forms a potential link between control of intra- and pericellular pH and tumor development. Therefore, the effects of oxidant stress were determined by the use of tert-butyl hydroperoxide (t-BOOH) on Na(+)/H(+) exchange in a biliary epithelial cancer cell line (Mz-Cha-1). The cells were exposed to the hydroperoxide and the rate of recovery from acidosis was determined by the use of the pH-sensitive fluorochrome 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF/AM). t-BOOH reduced Na(+)/H(+) exchange activity in a dose-dependent manner. At 4 mM t-BOOH, Na(+)/H(+) exchange activity was virtually absent. This was accompanied by an increase in cytotoxicity (MTT assay). Glutathione repletion and intracellular Ca(++) chelation partially restored the Na(+)/H(+) exchange activity. Hydroperoxide seemed neither to alter the intracellular signal transduction pathways (cAMP and Ca(++) oscillations) nor the membrane distribution of the exchanger (immunostaining). Decrease in Na(+)/H(+) exchange activity in this model of oxidant stress may represent an early perturbation of membrane function, and the functional integrity of Na(+)/H(+) exchange could therefore be dependent on the glutathione redox system.
机译：在胆管癌发生中，慢性炎症和氧化应激起关键作用。 Na（+）/ H（+）交换剂（NHE）形成控制细胞内和细胞外pH值与肿瘤发展之间的潜在联系。因此，氧化应激的影响是通过使用叔丁基氢过氧化物（t-BOOH）对胆管上皮癌细胞系（Mz-Cha-1）中Na（+）/ H（+）交换的影响来确定的。使细胞暴露于氢过氧化物，并通过使用pH敏感的荧光染料2'，7'-双（羧乙基）-5（6）-羧基荧光素乙酰氧基甲基酯（BCECF / AM）确定酸中毒的恢复率。 t-BOOH以剂量依赖性方式降低Na（+）/ H（+）交换活性。在4 mM t-BOOH时，实际上不存在Na（+）/ H（+）交换活性。这伴随着细胞毒性的增加（MTT测定）。谷胱甘肽的补充和细胞内Ca（++）螯合部分恢复Na（+）/ H（+）交换活动。氢过氧化物似乎既不改变细胞内信号转导途径（cAMP和Ca（++）振荡），也不改变交换器的膜分布（免疫染色）。在这种氧化应激模型中，Na（+）/ H（+）交换活性的降低可能代表膜功能的早期扰动，因此Na（+）/ H（+）交换的功能完整性可能取决于谷胱甘肽氧化还原系统。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号