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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structural studies of MIP synthase
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Structural studies of MIP synthase

机译：MIP合酶结构的研究

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摘要

The conversion of glucose 6-phosphate to 1-L-myo-inositol 1-phosphate (MIP) by 1-L-myo-inositol 1-phosphate synthase (MIP synthase) is the first committed and rate-limiting step in the de novo biosynthesis of inositol in all eukaryotes. The importance of inositol-containing molecules both as membrane components and as critical second messenger signal-transduction species make the function and regulation of this enzyme important for a host of biologically important cellular functions including proliferation, neurostimulation, secretion and contraction. MIP synthase has been overexpressed in Esherichia coli and purified to homogeneity by chromatographic methods. Two crystal forms of MIP synthase were obtained by the hanging-drop vapor-diffusion method. Native data sets for both crystal forms were collected in-house on a Rigaku R-AXIS IIC imaging-plate detector. Crystal form I belongs to space group C2, with unit-cell parameters a = 153.0, b = 96.6, c = 122.6 A, β = 126.4°, and diffracts to 2.5 A resolution. Crystal form II belongs to space group p2_1, with unit-cell parameters a = 94.5, b = 186.2, c = 86.5 A, β = 110.5°, and diffracts to 2.9 A resolution.
机译：葡萄糖6-phosphate的转换1-L-myo-inositol 1-phosphate (MIP)1-L-myo-inositol 1-phosphate合成酶(MIP)合酶)是第一个承诺病原新创生物合成的步骤肌醇在所有真核生物。inositol-containing分子既是膜组件和第二信使一样重要使功能和信号转导物种这种酶调节重要的主机生物学上重要的细胞功能包括增殖、神经刺激分泌和收缩。(在大肠杆菌和净化同质性的色谱方法。晶体形式的MIP合酶被通过的悬滴扩散的方法。晶体形式收集的数据集内部在Rigaku轴IIC imaging-plate探测器。C2,晶胞参数= 153.0,=96.6, c = 122.6,β= 126.4°,期刊2.5一项决议。空间群p2_1,晶胞参数=94.5, b = 186.2, c = 86.5,β= 110.5°,期刊到2.9一项决议。

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