β-Carbonic anhydrase from Pisum sativum: crystallization and preliminary X-ray analysis

Matthew S. Kimber; John R. Coleman; Emil F. Pai

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β-Carbonic anhydrase from Pisum sativum: crystallization and preliminary X-ray analysis

机译：β碳酸脱水酶从Pisum一:结晶和初步的x射线分析

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Recombinant β-carbonic anhydrase from the garden pea, Pisum sativum, was purified to homogeneity and crystallized. Crystals belong to the orthorhombic space group C222, with unit-cell parameters a = 136.3, b = 142.5, c = 201.4 A, α = β = γ = 90°. Crystals typically diffracted anisotropically, with a maximal resolution of 2.0 A in the strongest direction. The calculated Matthews parameter predicts approximately eight molecules in the asymmetric unit, consistent with previous reports of the molecule being an octamer. However, examination of the self-rotation function revealed no fourfold symmetry axis and multiple weak twofold axes perpendicular to the crystallographic c axis, indicating that the oligomerization arrangement is not that of a 422 octamer.

机译：重组β碳酸脱水酶的花园豌豆、Pisum一,是同质性的纯化和结晶。斜方晶系的空间群C222,晶胞参数= 136.3,= 142.5 b, c = 201.4,α=β=γ= 90°。anisotropically,最大分辨率为2.0最强的方向。马修斯参数预测大约8分子在不对称单位一致之前的报告作为一个分子八聚物。固有转动函数并没有发现四倍对称轴和多个弱两个轴垂直于晶体c轴,表明低聚安排不是422年的八聚物。

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《Acta crystallographica.Section D. Biological crystallography》 |2000年第7期|927-929|共3页
作者
Matthew S. Kimber; John R. Coleman; Emil F. Pai;
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作者单位

Department of Botany, University of Toronto, 25 Willcox Street, Toronto M5S 3B2, Canada;

Department of Molecular and Medical Genetics, University of Toronto, 1 King's College Circle, Toronto M5S 1A8, Canada;

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正文语种英语
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关键词
Peas; Carbonic Anhydrases; moleculesAxis of SymmetryhomogeneityX-ray analysisC-axiscrystalsAnisotropic magnetoresistanceCrystallization;

机译：analysisC-axiscrystalsAnisotropicmagnetoresistanceCrystallization;

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β-Carbonic anhydrase from Pisum sativum: crystallization and preliminary X-ray analysis

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