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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Suppression of Cell Invasion and Migration by Propofol Are Involved in Down-regulating Matrix Metalloproteinase-2 and p38 MAPK Signaling in A549 Human Lung Adenocarcinoma Epithelial Cells.
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Suppression of Cell Invasion and Migration by Propofol Are Involved in Down-regulating Matrix Metalloproteinase-2 and p38 MAPK Signaling in A549 Human Lung Adenocarcinoma Epithelial Cells.

机译：丙泊酚对细胞侵袭和迁移的抑制作用涉及下调A549人肺腺癌上皮细胞基质金属蛋白酶2和p38 MAPK信号传导。

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摘要

Metastasis is a major cause of death of patients with malignant tumors. Matrix metalloproteinases (MMPs) are important for the migration and invasion of various types of cancer cell. Propofol is a known anesthetic agent, widely used for short-term anesthesia and for longer-term sedation. Propofol inhibits the proliferation of a variety of tumor cells, but there is no available information regarding propofol-inhibited migration and invasion of tumor cells in vitro. In this study, we investigated the effects of propofol on the migration and invasion of human lung carcinoma A549 cells. Wound healing assay and Boyden chamber assays indicated that propofol inhibited the migration and invasion of A549 cells in vitro. Gelatin zymographic analysis showed the inhibitory effect of propofol on the activation of expression MMP-2. Western blot analysis also indicated that propofol suppressed the protein expiration of growth factor receptor-bound protein 2 (GRB2), Jun N-terminal kinases 1/2 (p-JNK1/2), p-p38, MMP-2 and MMP-9 in A549 cells. Results from real-time PCR assay also showed that propofol inhibited the mRNA gene expression of MMP-2, -7 and -9, and enhanced that of tissue inhibitor of metalloproteinase 1 (TIMP1) and TIMP2 in A549 cells. Taken together, these data show that propofol inhibits MMP-2 and -9 mRNA and protein expressions, resulting in suppression of lung cancer cell invasion and migration in vitro.
机译：转移是恶性肿瘤患者死亡的主要原因。基质金属蛋白酶（MMP）对于各种类型的癌细胞的迁移和侵袭很重要。丙泊酚是一种已知的麻醉剂，广泛用于短期麻醉和长期镇静。异丙酚可抑制多种肿瘤细胞的增殖，但尚无关于异丙酚在体外抑制肿瘤细胞迁移和侵袭的信息。在这项研究中，我们调查了异丙酚对人肺癌A549细胞迁移和侵袭的影响。伤口愈合试验和博伊登室试验表明，异丙酚在体外可抑制A549细胞的迁移和侵袭。明胶酶谱分析表明丙泊酚对MMP-2表达的激活具有抑制作用。 Western印迹分析还表明，异丙酚可抑制生长因子受体结合蛋白2（GRB2），Jun N端激酶1/2（p-JNK1 / 2），p-p38，MMP-2和MMP-9的蛋白过期在A549细胞中实时PCR检测结果还显示，丙泊酚抑制了A549细胞中MMP-2，-7和-9的mRNA基因表达，并增强了金属蛋白酶1（TIMP1）和TIMP2的组织抑制剂。综上所述，这些数据表明丙泊酚抑制MMP-2和-9 mRNA和蛋白质表达，从而抑制了肺癌细胞在体外的侵袭和迁移。

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