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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification and crystallization of recombinant human TRAIL.
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Expression, purification and crystallization of recombinant human TRAIL.

机译：的表达、纯化和结晶重组人类的踪迹。

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摘要

TRAIL (also known as Apo-2L) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to the death-signalling receptors on the cell membrane. Normal cells are resistant to TRAIL, owing to the expression of decoy receptors which lack functional death domains and antagonize TRAIL-induced apoptosis. Soluble and functional human TRAIL, expressed in Escherichia coli and refolded into a functional form, has been crystallized. The crystals belong to space group P63 with unit-cell dimensions a = b = 65.61, c = 131. 70 A. The asymmetric unit contains two molecules of TRAIL, with a crystal volume per protein mass (Vm) of 2.41 A3 Da-1 and a solvent content of about 42% by volume. A native and a platinum-derivative data set to 2.8 and 3.5 A resolution, respectively, were obtained from frozen crystals. Structure determination by a combined molecular replacement and isomorphous replacement method is in progress.
机译：跟踪(也称为Apo-2L)属于肿瘤家人和诱导坏死因子(TNF)细胞因子快速在各种肿瘤细胞凋亡线在death-signalling绑定细胞膜上的受体。耐,由于表达的诱饵受体缺乏功能性死亡域和对抗TRAIL-induced细胞凋亡。溶性和功能性人工痕迹,表达大肠杆菌和复合功能形式,结晶。空间群P63和单胞尺寸=b = 65.61, c = 131。包含两个分子的踪迹,晶体体积/蛋白质质量(Vm) 2.41 A3 Da-1和溶剂含量约42%。本地和platinum-derivative数据设置为2.8分别和3.5决议从冰冻的晶体。结合分子置换和同形替代方法是在进步。

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